ArticleGenetics1999
Genetic identification of three ABC transporters as essential elements for nitrate respiration in Haloferax volcanii.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Studying the long-term adaptation ofFrontiers in microbiology · 2025Article
- Integrated Analysis of Molybdenum Nutrition and Nitrate Metabolism in Strawberry.Frontiers in plant science · 2020Article
- Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.Microbiology and molecular biology reviews : MMBR · 2014Review
- Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism.Molecular & cellular proteomics : MCP · 2014Article
- The complete genome sequence of Natrinema sp. J7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements.PloS one · 2012Article
- Functional genomic and advanced genetic studies reveal novel insights into the metabolism, regulation, and biology of Haloferax volcanii.Archaea (Vancouver, B.C.) · 2011Article
- Nitrogen metabolism in haloarchaea.Saline systems · 2008Article
- An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Insights into ABC transport in archaea.Journal of bioenergetics and biomembranes · 2004Review
- The unique features of glycolytic pathways in Archaea.The Biochemical journal · 2003Review
- Functional role for a 2-oxo acid dehydrogenase in the halophilic archaeon Haloferax volcanii.Journal of bacteriology · 2002Article
- What archaea have to tell biologists.Genetics · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
More than 40 nitrate respiration-deficient mutants of Haloferax volcanii belonging to three different phenotypic classes were isolated. All 15 mutants of the null phenotype were complemented with a genomic library of the wild type. Wild-type copies of mutated genes were recovered from complemented mutants using two different approaches. The DNA sequences of 13 isolated fragments were determined. Five fragments were found to overlap; therefore nine different genomic regions containing genes essential for nitrate respiration could be identified. Three genomic regions containing genes coding for subunits of ABC transporters were further characterized. In two cases, genes coding for an ATP-binding subunit and a permease subunit were clustered and overlapped by four nucleotides. The third gene for a permease subunit had no additional ABC transporter gene in proximity. One ABC transporter was found to be glucose specific. The mutant reveals that the ABC transporter solely mediates anaerobic glucose transport. Based on sequence similarity, the second ABC transporter is proposed to be molybdate specific, explaining its essential role in nitrate respiration. The third ABC transporter is proposed to be anion specific. Genome sequencing has shown that ABC transporters are widespread in Archaea. Nevertheless, this study represents only the second example of a functional characterization.
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