ArticleThe Journal of cell biology1999
Roles for alpha(2)p24 and COPI in endoplasmic reticulum cargo exit site formation.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 92 citations in OpenAlex.
- TMED family genes and their roles in human diseases.International journal of medical sciences · 2023Pooled it
- TMED9 coordinates the clearance of misfolded GPI-anchored proteins out of the ER and into the Golgi.PLoS biology · 2025Article
- p24-Tango1 interactions ensure ER-Golgi interface stability and efficient transport.The Journal of cell biology · 2024Article
- The Sar1 GTPase is dispensable for COPII-dependent cargo export from the ER.Cell reports · 2023Article
- The many hats of transmembrane emp24 domain protein TMED9 in secretory pathway homeostasis.Frontiers in cell and developmental biology · 2022Review
- COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers.The Journal of cell biology · 2021Article
- BPIFB3 interacts with ARFGAP1 and TMED9 to regulate non-canonical autophagy and RNA virus infection.Journal of cell science · 2021Article
- The protein secretion modulator TMED9 drives CNIH4/TGFα/GLI signaling opposing TMED3-WNT-TCF to promote colon cancer metastases.Oncogene · 2019Article
- Article
- p24 family proteins: key players in the regulation of trafficking along the secretory pathway.Protoplasma · 2016Review
- Characterization of Gene Expression Patterns among Artificially Developed Cancer Stem Cells Using Spherical Self-Organizing Map.Cancer informatics · 2016Article
- ARF1 and SAR1 GTPases in endomembrane trafficking in plants.International journal of molecular sciences · 2013Review
- Putative p24 complexes in Arabidopsis contain members of the delta and beta subfamilies and cycle in the early secretory pathway.Journal of experimental botany · 2013Article
- Coupled transport of Arabidopsis p24 proteins at the ER-Golgi interface.Journal of experimental botany · 2012Article
- The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta.Developmental biology · 2010Article
- The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi.Molecular biology of the cell · 2008Article
- The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus.The Journal of cell biology · 2008Article
- Topology of molecular machines of the endoplasmic reticulum: a compilation of proteomics and cytological data.Histochemistry and cell biology · 2008Review
- The secretory system of Arabidopsis.The arabidopsis book · 2008Article
- Disparate effects of p24alpha and p24delta on secretory protein transport and processing.PloS one · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A two-step reconstitution system for the generation of ER cargo exit sites from starting ER-derived low density microsomes (LDMs; 1.17 g/cc) is described. The first step is mediated by the hydrolysis of Mg(2+)ATP and Mg(2+)GTP, leading to the formation of a transitional ER (tER) with the soluble cargo albumin, transferrin, and the ER-to-Golgi recycling membrane proteins alpha(2)p24 and p58 (ERGIC-53, ER-Golgi intermediate compartment protein) enriched therein. Upon further incubation (step two) with cytosol and mixed nucleotides, interconnecting smooth ER tubules within tER transforms into vesicular tubular clusters (VTCs). The cytosolic domain of alpha(2)p24 and cytosolic COPI coatomer affect VTC formation. This is deduced from the effect of antibodies to the COOH-terminal tail of alpha(2)p24, but not of antibodies to the COOH-terminal tail of calnexin on this reconstitution, as well as the demonstrated recruitment of COPI coatomer to VTCs, its augmentation by GTPgammaS, inhibition by Brefeldin A (BFA), or depletion of beta-COP from cytosol. Therefore, the p24 family member, alpha(2)p24, and its cytosolic coat ligand, COPI coatomer, play a role in the de novo formation of VTCs and the generation of ER cargo exit sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.