Evidence map›Paper›PMID 10427014›Full record

ArticleApplied and environmental microbiology1999

The spheroplast lysis assay for yeast in microtiter plate format.

R Ovalle, M Spencer, M Thiwanont, P N Lipke

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. A histone deacetylase-dependent screen in yeast.Bioorganic & medicinal chemistry · 2010
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

R OvalleDepartment of Biological Sciences and Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, New York 10021, USA.
M Spencer
M Thiwanont
P N Lipke

Funding

WILD TYPE &MUTANT P53 INTERACTIONS WITH HIV LTR DNAG12RR003037 · NCRR · HUNTER COLLEGE · PI DOTTIN, ROBERT P · 1985 to 2011
$27.6M
MOLECULAR GENETICS OF YEAST SEXUAL AGGLUTININSR01GM047176 · NIGMS · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI KURJAN, JANET A. · 1991 to 1999
–
NCRR NIH HHS G12 RR003037NCRR NIH HHS RR03037NIGMS NIH HHS 1RO1-GM47176
6 · The paper itself

Abstract

A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for drug susceptibility and for analyzing mutant phenotypes.

Indexed as

Cell WallHydrolasesMycologyReproducibility of ResultsSaccharomyces cerevisiaeSpheroplastsHydrolaseszymolyase

Identifiers

PMID10427014
PMCPMC91499

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.