ArticleBiophysical journal1999
The temperature sensitivity of miniature endplate currents is mostly governed by channel gating: evidence from optimized recordings and Monte Carlo simulations.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Microphysiological Modeling of the Structure and Function of Neuromuscular Transmitter Release Sites.Frontiers in synaptic neuroscience · 2022Review
- Stochastic shielding and edge importance for Markov chains with timescale separation.PLoS computational biology · 2018Article
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- Mechanisms of the inhibition of endplate acetylcholine receptors by antiseptic chlorhexidine (experiments and models).Naunyn-Schmiedeberg's archives of pharmacology · 2009Article
- FAST MONTE CARLO SIMULATION METHODS FOR BIOLOGICAL REACTION-DIFFUSION SYSTEMS IN SOLUTION AND ON SURFACES.SIAM journal on scientific computing : a publication of the Society for Industrial and Applied Mathematics · 2008Article
- Modeling study of mecamylamine block of muscle type acetylcholine receptors.European biophysics journal : EBJ · 2008Article
- Responses of rat P2X2 receptors to ultrashort pulses of ATP provide insights into ATP binding and channel gating.The Journal of general physiology · 2007Article
- Acceleration of AMPA receptor kinetics underlies temperature-dependent changes in synaptic strength at the rat calyx of Held.The Journal of physiology · 2007Article
- An efficient stochastic diffusion algorithm for modeling second messengers in dendrites and spines.Journal of neuroscience methods · 2006Article
- Article
- Evidence for ectopic neurotransmission at a neuronal synapse.Science (New York, N.Y.) · 2005Article
- THE VIRTUAL INSTRUMENT: SUPPORT FOR GRID-ENABLED MCELL SIMULATIONS.The international journal of high performance computing applications · 2004Article
- Mutation causing severe myasthenia reveals functional asymmetry of AChR signature cystine loops in agonist binding and gating.The Journal of clinical investigation · 2003Article
- The effects of temperature on vesicular supply and release in autaptic cultures of rat and mouse hippocampal neurons.The Journal of physiology · 2002Article
- Novel delta subunit mutation in slow-channel syndrome causes severe weakness by novel mechanisms.Annals of neurology · 2002Article
- Fine-tuning an auditory synapse for speed and fidelity: developmental changes in presynaptic waveform, EPSC kinetics, and synaptic plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The temperature dependence of miniature endplate current (MEPC) amplitude (A(c)), 20-80% rise time (t(r)), and 90-33% fall-time (t(f)) was determined for lizard (Anolis carolinensis) intercostal muscle using broadband extracellular (EC) and voltage clamp (VC) recordings. Voltage clamp methods were optimized for the fast MEPC rising phase using custom electronics. From 0-43 degrees C, A(c) increased by approximately 4.2-fold, while t(r) and t(f) decreased by approximately 3.6- and approximately 9.5-fold, respectively. Arrhenius plots were smoothly curved, with small apparent Q(10) (A(c)) or (Q(10))(-1) (t(r) and t(f)) values mostly well below 2.0. Nearly identical extracellular and voltage clamp results ruled out measurement artifacts, even for the shortest t(r) values (<60 microseconds). Monte Carlo simulation of MEPCs showed that a single underlying rate cannot determine the observed temperature dependence. To quantitatively reproduce the experimental t(f) results, a minimal model required activation energies of 46.0 (Q(10) approximately 2.0) and 63.6 (Q(10) approximately 2.5) kJ mol(-1) for channel opening and closing, respectively, and accounted for most of the observed changes in A(c) and t(r) as well. Thus, relatively large but offsetting temperature sensitivities of channel gating mostly govern and minimize the temperature dependence of MEPCs, preserving the safety factor for neuromuscular transmission. Additional temperature-sensitive parameters that could fine-tune the minimal model are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.