ArticleBiophysical journal1999
Tension of membranes expressing the hemagglutinin of influenza virus inhibits fusion.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Respiratory syncytial virus fuses with plasma membrane to infect primary cultures of bronchial epithelial cells.Frontiers in microbiology · 2025Article
- Article
- Chloroplast membrane lipid remodeling protects against dehydration by limiting membrane fusion and distortion.Plant physiology · 2022Article
- Membrane tension may define the deadliest virus infection.Colloid and interface science communications · 2021Article
- Membrane tension and membrane fusion.Current opinion in structural biology · 2015Review
- Resolving single membrane fusion events on planar pore-spanning membranes.Scientific reports · 2015Article
- Wild-type and mutant hemagglutinin fusion peptides alter bilayer structure as well as kinetics and activation thermodynamics of stalk and pore formation differently: mechanistic implications.Biophysical journal · 2013Article
- Line-tension controlled mechanism for influenza fusion.PloS one · 2012Article
- Cytoskeleton reorganization in influenza hemagglutinin-initiated syncytium formation.Biochimica et biophysica acta · 2009Article
- Fusion induced by a class II viral fusion protein, semliki forest virus E1, is dependent on the voltage of the target cell.Journal of virology · 2007Article
- Time-resolved imaging of HIV-1 Env-mediated lipid and content mixing between a single virion and cell membrane.Molecular biology of the cell · 2005Article
- Properties and structures of the influenza and HIV fusion peptides on lipid membranes: implications for a role in fusion.Biophysical journal · 2005Article
- Osmotic and curvature stress affect PEG-induced fusion of lipid vesicles but not mixing of their lipids.Biophysical journal · 2002Article
- A quantitative model for membrane fusion based on low-energy intermediates.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- A point mutation in the transmembrane domain of the hemagglutinin of influenza virus stabilizes a hemifusion intermediate that can transit to fusion.Molecular biology of the cell · 2000Article
- Dynamics of fusion pores connecting membranes of different tensions.Biophysical journal · 2000Article
- Imaging constitutive exocytosis with total internal reflection fluorescence microscopy.The Journal of cell biology · 2000Article
- The lipid-anchored ectodomain of influenza virus hemagglutinin (GPI-HA) is capable of inducing nonenlarging fusion pores.Molecular biology of the cell · 2000Article
- Membrane fusion mediated by coiled coils: a hypothesis.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The effects of membrane tension on fusion between cells expressing the hemagglutinin (HA) of influenza virus and red blood cells were studied by capacitance measurements. Inflation of an HA-expressing cell was achieved by applying a positive hydrostatic pressure to its interior through a patch-clamp pipette in the whole-cell configuration. Inflating cells to the maximum extent possible without lysis created a membrane tension and completely inhibited low-pH-induced fusion at room temperature. Fully inflated cells that were subsequently deflated to normal size resumed the ability to fuse in response to low pH. At the higher temperature of 32 degrees C, fusion conditions were sufficiently optimal that full inflation did not hinder fusion, and once formed, pores enlarged more rapidly than those of never inflated cells. It is suggested that under fusogenic conditions HA causes the formation of a dimple within the membrane in which it resides, and that membrane tension hinders fusion by preventing the formation of dimples. Because dimpling bends the bilayer portion of bound membranes so that they come into intimate contact, the damping of dimpling would suppress this initial step in the fusion process.
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