ArticleBiophysical journal1999
Electron spin resonance characterization of liquid ordered phase of detergent-resistant membranes from RBL-2H3 cells.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 117 citations in OpenAlex.
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- Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes.Scientific reports · 2021Article
- Critical Negatively Charged Residues Are Important for the Activity of SARS-CoV-1 and SARS-CoV-2 Fusion Peptides.bioRxiv : the preprint server for biology · 2021Article
- SARS-CoV-2 Fusion Peptide has a Greater Membrane Perturbating Effect than SARS-CoV with Highly Specific Dependence on CaJournal of molecular biology · 2021Article
- Regulating Lipid Composition Rationalizes Acyl Tail Saturation Homeostasis in Ectotherms.Biophysical journal · 2020Article
- Direct Prediction of EPR Spectra from Lipid Bilayers: Understanding Structure and Dynamics in Biological Membranes.Chemphyschem : a European journal of chemical physics and physical chemistry · 2018Article
- The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner.Journal of molecular biology · 2017Article
- Roles for lipid heterogeneity in immunoreceptor signaling.Biochimica et biophysica acta · 2016Review
- Protective role of the dynamin inhibitor Dynasore against the cholesterol-dependent cytolysin of Trueperella pyogenes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Cholesterol enhances surface water diffusion of phospholipid bilayers.The Journal of chemical physics · 2014Article
- Membrane interaction of retroviral Gag proteins.Frontiers in microbiology · 2014Review
- Colocalization of the ganglioside G(M1) and cholesterol detected by secondary ion mass spectrometry.Journal of the American Chemical Society · 2013Article
- Dynamics and ordering of lipid spin-labels along the coexistence curve of two membrane phases: an ESR study.Chemistry and physics of lipids · 2012Article
- Phases and domains in sphingomyelin-cholesterol membranes: structure and properties using EPR spin-labeling methods.European biophysics journal : EBJ · 2012Article
- 2D-ELDOR study of heterogeneity and domain structure changes in plasma membrane vesicles upon cross-linking of receptors.The journal of physical chemistry. B · 2011Article
- Phase-separation and domain-formation in cholesterol-sphingomyelin mixture: pulse-EPR oxygen probing.Biophysical journal · 2011Article
- Cannabinoid receptor signalling in neurodegenerative diseases: a potential role for membrane fluidity disturbance.British journal of pharmacology · 2011Review
- Perfringolysin O association with ordered lipid domains: implications for transmembrane protein raft affinity.Biophysical journal · 2010Article
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6 authors at 1 institution in 1 country.
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Abstract
The dynamic structure of detergent-resistant membranes (DRMs) isolated from RBL-2H3 cells was characterized using two different acyl chain spin-labeled phospholipids (5PC and 16PC), a headgroup labeled sphingomyelin (SM) analog (SD-Tempo) and a spin-labeled cholestane (CSL). It was shown, by comparison to dispersions of SM, dipalmitoylphosphatidylcholine (DPPC), and DPPC/cholesterol of molar ratio 1, that DRM contains a substantial amount of liquid ordered phase: 1) The rotational diffusion rates (R( perpendicular)) of 16PC in DRM between -5 degrees C and 45 degrees C are nearly the same as those in molar ratio DPPC/Chol = 1 dispersions, and they are substantially greater than R( perpendicular) in pure DPPC dispersions in the gel phase studied above 20 degrees C; 2) The order parameters (S) of 16PC in DRM at temperatures above 4 degrees C are comparable to those in DPPC/Chol = 1 dispersions, but are greater than those in DPPC dispersions in both the gel and liquid crystalline phases. 3) Similarly, R( perpendicular) for 5PC and CSL in DRM is greater than in pure SM dispersions in the gel phase, and S for these labels in DRM is greater than in the SM dispersions in both the gel and liquid crystalline phases. 4) R( perpendicular) of SD-Tempo in DRM is greater than in dispersions of SM in both gel and liquid phases, consistent with the liquid-like mobility in the acyl chain region in DRM. However, S of SD-Tempo in DRM is substantially less than that of this spin label in SM in gel and liquid crystalline phases (in absolute values), indicating that the headgroup region in DRMs is less ordered than in pure SM. These results support the hypothesis that plasma membranes contain DRM domains with a liquid ordered phase that may coexist with a liquid crystalline phase. There also appears to be a coexisting region in DRMs in which the chain labels 16PC and 5PC are found to cluster. We suggest that other biological membranes containing high concentrations of cholesterol also contain a liquid ordered phase.
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