ArticleBiophysical journal1999
Actin protofilament orientation at the erythrocyte membrane.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Feisty filaments: actin dynamics in the red blood cell membrane skeleton.Current opinion in hematology · 2016Review
- Organization of FtsZ filaments in the bacterial division ring measured from polarized fluorescence microscopy.Biophysical journal · 2013Article
- Nanomechanics of multiple units in the erythrocyte membrane skeletal network.Annals of biomedical engineering · 2010Article
- Fluorescence polarization/anisotropy in diagnostics and imaging.Chemical reviews · 2010Review
- Spectrin folding versus unfolding reactions and RBC membrane stiffness.Biophysical journal · 2008Article
- A hybrid model for erythrocyte membrane: a single unit of protein network coupled with lipid bilayer.Biophysical journal · 2007Article
- Topographical pattern dynamics in passive adhesion of cell membranes.Biophysical journal · 2004Article
- Deformation-enhanced fluctuations in the red cell skeleton with theoretical relations to elasticity, connectivity, and spectrin unfolding.Biophysical journal · 2001Article
- Elastic thickness compressibilty of the red cell membrane.Biophysical journal · 2001Article
- Actin protofilament orientation in deformation of the erythrocyte membrane skeleton.Biophysical journal · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The short actin filaments in the erythrocyte's membrane skeleton are shown to be largely oriented tangent to the lipid bilayer. Actin "proto"-filaments have previously been described as junctional centers intertriangulated by spectrin; however, the protofilaments may simultaneously serve as pinning centers between the network and the overlying bilayer. The latter function now seems of particular importance because near-normal network assembly has been reported with transgenic mouse sphero-erythrocytes that lack the primary linkage protein Band 3. To assess possible physical constraints on actin protofilaments in intact membranes, fluorescence polarization microscopy (FPM) has been used to study rhodamine phalloidin-labeled red cell ghosts. A basis for interpreting FPM images of cells is provided by FPM applied to isolated actin filaments. These are labeled with the same rhodamine probes and imaged at various orientations with respect to the polarizers, including filament orientations perpendicular to the image plane. High aperture and fluorophore conjugation effects are found to be minimal, enabling development of a simple, semi-empirical model which indicates that protofilaments are generally within approximately 20 degrees of the membrane tangent plane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.