ArticleBiophysical journal1999
Direct measures of large, anisotropic strains in deformation of the erythrocyte cytoskeleton.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- The stress-free state of human erythrocytes: Data-driven inference of a transferable RBC model.Biophysical journal · 2023Article
- Review
- Development of Mechanical Stability in Late-Stage Embryonic Erythroid Cells: Insights From Fluorescence Imaged Micro-Deformation Studies.Frontiers in physiology · 2021Article
- Role of SIRT1-mediated mitochondrial and Akt pathways in glioblastoma cell death induced by Cotinus coggygria flavonoid nanoliposomes.International journal of nanomedicine · 2015Article
- Dynamics of red blood cells in microporous membranes.Biomicrofluidics · 2014Article
- Stress-free state of the red blood cell membrane and the deformation of its skeleton.Cellular & molecular biology letters · 2012Article
- Native ultrastructure of the red cell cytoskeleton by cryo-electron tomography.Biophysical journal · 2011Article
- Nanocarriers for vascular delivery of antioxidants.Nanomedicine (London, England) · 2011Review
- Targeting delivery of drugs in the vascular system.International journal of transport phenomena · 2011Article
- Nanomechanics of multiple units in the erythrocyte membrane skeletal network.Annals of biomedical engineering · 2010Article
- Polymeric carriers: role of geometry in drug delivery.Expert opinion on drug delivery · 2008Review
- Spectrin-level modeling of the cytoskeleton and optical tweezers stretching of the erythrocyte.Biophysical journal · 2005Article
- Estimating the sensitivity of mechanosensitive ion channels to membrane strain and tension.Biophysical journal · 2004Article
- Echinocyte shapes: bending, stretching, and shear determine spicule shape and spacing.Biophysical journal · 2002Article
- Deformation-enhanced fluctuations in the red cell skeleton with theoretical relations to elasticity, connectivity, and spectrin unfolding.Biophysical journal · 2001Article
- Actin protofilament orientation in deformation of the erythrocyte membrane skeleton.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The erythrocyte's spectrin-actin membrane skeleton is directly shown to be capable of sustaining large, anisotropic strains. Photobleaching of an approximately 1-micrometer stripe in rhodamine phalloidin-labeled actin appears stable up to at least 37 degrees C, and is used to demonstrate large in-surface stretching during elastic deformation of the skeleton. Principal extension or stretch ratios of at least approximately 200% and contractions down to approximately 40%, both referenced to an essentially undistorted cell, are visually demonstrated in micropipette-imposed deformation. Such anisotropic straining is seen to be consistent at a qualitative level with now classic analyses (Evans. 1973. Biophys. J. 13:941-954) and is generally nonhomogeneous though axisymmetric down to the submicron scale. Local, direct measurements of stretching prove quantitatively consistent (within approximately 10%) with integrated estimates that are based simply on a measured relative density distribution of actin. The measurements are also in close agreement with direct computation of mean spectrin chain extension in full statistical mechanical simulations of a coarse-grained network held in a micropipette. Finally, as a cell thermally fragments near approximately 48 degrees C, the patterned photobleaching demonstrates a destructuring of the surface network in a process that is more readily attributable to transitions in spectrin than in F-actin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.