ArticleBiophysical journal1999
Practical limits on the maximal speed of solution exchange for patch clamp experiments.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Fast functional mapping of ligand-gated ion channels.Communications biology · 2023Article
- The kinetic properties of the α3 rat glycine receptor make it suitable for mediating fast synaptic inhibition.The Journal of physiology · 2013Article
- Sub-millisecond ligand probing of cell receptors with multiple solution exchange.Nature protocols · 2013Article
- The α1K276E startle disease mutation reveals multiple intermediate states in the gating of glycine receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Article
- Refractive-index-based screening of membrane-protein-mediated transfer across biological membranes.Biophysical journal · 2010Article
- Mechanical and energetic consequences of HCM-causing mutations.Journal of cardiovascular translational research · 2009Review
- Achieving synaptically relevant pulses of neurotransmitter using PDMS microfluidics.Journal of neuroscience methods · 2009Article
- Responses of rat P2X2 receptors to ultrashort pulses of ATP provide insights into ATP binding and channel gating.The Journal of general physiology · 2007Article
- Tension generation and relaxation in single myofibrils from human atrial and ventricular myocardium.Pflugers Archiv : European journal of physiology · 2007Article
- A chemical waveform synthesizer.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Sarcomeric determinants of striated muscle relaxation kinetics.Pflugers Archiv : European journal of physiology · 2005Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying ligand-gated ion channels often requires the ability to change solutions quickly. Using finite element models, I have examined the practical limitations of how fast solutions can be exchanged on an outside-out patch using a dual stream switcher. The primary factors controlling the speed of response are the flow velocity, proximity of the patch to the exit ports, the width of the partition between the two streams, the velocity with which the streams can be moved across the patch, and the viscosity of the solutions. The practical limit seems to be a rise time of approximately 20 microseconds. The rate-limiting step is the velocity of the (usually piezo) motor that translates the streams across the patch. Increasing the perfusate viscosity improves speed by slowing dissipation of the concentration gradients. A flow switcher can also be used for bipolar temperature jumps with a rise time of approximately 100 microseconds.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.