ArticleGenes & development1999
A GATA-2/estrogen receptor chimera functions as a ligand-dependent negative regulator of self-renewal.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 87 citations in OpenAlex.
- Article
- GATA2 hypomorphism induces chronic myelomonocytic leukemia in mice.Cancer science · 2019Article
- Functional and molecular characterization of mouse Gata2-independent hematopoietic progenitors.Blood · 2016Article
- Histone methyltransferase Setd8 represses Gata2 expression and regulates erythroid maturation.Molecular and cellular biology · 2015Article
- Haematopoietic and immune defects associated with GATA2 mutation.British journal of haematology · 2015Review
- Review
- Dynamic analysis of gene expression and genome-wide transcription factor binding during lineage specification of multipotent progenitors.Cell stem cell · 2013Article
- The biochemistry of hematopoietic stem cell development.Biochimica et biophysica acta · 2013Review
- GATA-2 mediated regulation of normal hematopoietic stem/progenitor cell function, myelodysplasia and myeloid leukemia.The international journal of biochemistry & cell biology · 2012Review
- A single cis element maintains repression of the key developmental regulator Gata2.PLoS genetics · 2010Article
- FOG-1-mediated recruitment of NuRD is required for cell lineage re-enforcement during haematopoiesis.The EMBO journal · 2010Article
- Expression levels of histone deacetylases determine the cell fate of hematopoietic progenitors.The Journal of biological chemistry · 2009Article
- Article
- Review
- PU.1 inhibits the erythroid program by binding to GATA-1 on DNA and creating a repressive chromatin structure.The EMBO journal · 2005Article
- An Oct-1 binding site mediates activation of the gata2 promoter by BMP signaling.Nucleic acids research · 2005Article
- GATA-2 plays two functionally distinct roles during the ontogeny of hematopoietic stem cells.The Journal of experimental medicine · 2004Article
- Jun blockade of erythropoiesis: role for repression of GATA-1 by HERP2.Molecular and cellular biology · 2004Article
- Cross talk between retinoic acid signaling and transcription factor GATA-2.Molecular and cellular biology · 2004Article
- Molecular signatures of self-renewal, differentiation, and lineage choice in multipotential hemopoietic progenitor cells in vitro.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
The transcription factor GATA-2 is expressed in hematopoietic stem and progenitor cells and is functionally implicated in their survival and proliferation. We have used estrogen and tamoxifen-inducible forms of GATA-2 to modulate the levels of GATA-2 in the IL-3-dependent multipotential hematopoietic progenitor cell model FDCP mix. Ligand-dependent induction of exogenous GATA-2 activity did not rescue cells deprived of IL-3 from apoptosis. However, induction of GATA-2 activity in cells cultured in IL-3 blocked factor-dependent self-renewal but not factor-dependent survival: Cells undergo cell cycle arrest and cease proliferating but do not apoptose. This was accompanied by differentiation down the monocytic and granulocytic pathways. Differentiation occurred in the presence of IL-3 and did not require addition of exogenous differentiation growth factors such as G-CSF or GM-CSF normally required to induce granulomonocytic differentiation of FDCP-mix cells. Conversely, EPO-dependent erythroid differentiation was inhibited by GATA-2 activation. These biological effects were obtained with levels of exogenous GATA-2 representing less than twofold increases over endogenous GATA-2 levels and were not observed in cells overexpressing GATA-1/ER. Similar effects on proliferation and differentiation were also observed in primary progenitor cells, freshly isolated from murine bone marrow and transduced with a GATA-2/ER-containing retrovirus. Taken together, these data suggest that threshold activities of GATA-2 in hematopoietic progenitor cells are a critical determinant in influencing self-renewal versus differentiation outcomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.