ArticleThe Journal of physiology1999
The role of Ca2+-activated K+ channel spliced variants in the tonotopic organization of the turtle cochlea.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 104 citations in OpenAlex.
- Reproductive-state dependent changes in inner ear gene expression in female plainfin midshipman fish.PloS one · 2026Article
- Alternative splicing in shaping the molecular landscape of the cochlea.Frontiers in cell and developmental biology · 2023Review
- Inhibition of a transcriptional repressor rescues hearing in a splicing factor-deficient mouse.Life science alliance · 2020Article
- Diverse Mechanisms of Sound Frequency Discrimination in the Vertebrate Cochlea.Trends in neurosciences · 2020Review
- Effects of Single Nucleotide Polymorphisms in HumanFrontiers in molecular neuroscience · 2019Article
- Alternative splicing of inner-ear-expressed genes.Frontiers of medicine · 2016Review
- Multilevel regulation: Controlling BK channels in central clock neurons.The Journal of general physiology · 2013Article
- Phosphorylation of a constitutive serine inhibits BK channel variants containing the alternate exon "SRKR".The Journal of general physiology · 2013Article
- Songbird frequency selectivity and temporal resolution vary with sex and season.Proceedings. Biological sciences · 2013Article
- CDK5 interacts with Slo and affects its surface expression and kinetics through direct phosphorylation.American journal of physiology. Cell physiology · 2012Article
- Alternatively spliced domains interact to regulate BK potassium channel gating.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Identification and quantification of full-length BK channel variants in the developing mouse cochlea.Journal of neuroscience research · 2011Article
- Somatic motility and hair bundle mechanics, are both necessary for cochlear amplification?Hearing research · 2011Article
- β4-subunit increases Slo responsiveness to physiological Ca2+ concentrations and together with β1 reduces surface expression of Slo in hair cells.American journal of physiology. Cell physiology · 2011Article
- Neuronal fast activating and meningeal silent modulatory BK channel splice variants cloned from rat.Pflugers Archiv : European journal of physiology · 2011Article
- Cloning and distribution of Ca2+-activated K+ channels in lobster Panulirus interruptus.Neuroscience · 2010Article
- Highly specific alternative splicing of transcripts encoding BK channels in the chicken's cochlea is a minor determinant of the tonotopic gradient.Molecular and cellular biology · 2010Article
- Developmental expression of BK channels in chick cochlear hair cells.BMC developmental biology · 2009Article
- Regulation of the epithelial sodium channel [ENaC] in kidneys of salt-sensitive Dahl rats: insights on alternative splicing.International archives of medicine · 2009Article
- Calcium-activated potassium (BK) channels are encoded by duplicate slo1 genes in teleost fishes.Molecular biology and evolution · 2009Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
1. Turtle auditory hair cells contain multiple isoforms of the pore-forming alpha-subunit of the large-conductance Ca2+-activated K+ (KCa) channel due to alternative splicing at two sites. Six splice variants were studied by expression in Xenopus oocytes. 2. The isoforms possessed differences in apparent Ca2+ sensitivity and kinetics. The lowest Ca2+ sensitivity was observed in a novel variant resulting from a 26 amino acid deletion around one of the splice sites. 3. Co-expression of a bovine beta-subunit slowed the current relaxation 10-fold compared with channels formed from alpha-subunits alone but preserved the original order of kinetic differences. The beta-subunit also increased the Ca2+ sensitivity of isoforms to bring them nearer the range of sensitivity of the native KCa channels of the hair cell. 4. With channels formed from alpha-subunits or alpha + beta-subunits, the half-activation voltage in a fixed Ca2+ concentration, and the time constant of the current relaxation, varied linearly with the combined size of the insertions/deletions at the splice sites. 5. Experiments in which the beta/alpha concentration ratio was varied indicated that the beta-subunit exerts an all-or-none effect on the Ca2+ sensitivity and kinetics of the channel. 6. Co-expression of an avian beta2-subunit had effects on kinetics and Ca2+ sensitivity of several alpha-isoforms which were qualitatively similar to those produced by the bovine beta-subunit. 7. We conclude that differential expression of alternatively spliced alpha-subunit variants and a non-uniform distribution of a beta-subunit can produce a range of KCa channel properties needed to explain the tonotopic organization of the turtle cochlea.
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