ArticleJournal of bacteriology1999
The Streptomyces peucetius dpsC gene determines the choice of starter unit in biosynthesis of the daunorubicin polyketide.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 62 citations in OpenAlex.
- A unique dual acyltransferase system shared in the polyketide chain initiation of kidamycinone and rubiflavinone biosynthesis.Frontiers in microbiology · 2023Article
- A BioBricks Metabolic Engineering Platform for the Biosynthesis of Anthracyclinones inACS synthetic biology · 2022Article
- Targeted Genome Mining-From Compound Discovery to Biosynthetic Pathway Elucidation.Microorganisms · 2020Article
- Biosynthesis of Polyketides inMicroorganisms · 2019Review
- An Oxetane-Based Polyketide Surrogate To Probe Substrate Binding in a Polyketide Synthase.Journal of the American Chemical Society · 2018Article
- Structural Insights into Anthranilate Priming during Type II Polyketide Biosynthesis.ACS chemical biology · 2016Article
- Methylation-dependent acyl transfer between polyketide synthase and nonribosomal peptide synthetase modules in fungal natural product biosynthesis.Organic letters · 2014Article
- Discovery of the lomaiviticin biosynthetic gene cluster inTetrahedron · 2014Article
- Lomaiviticin biosynthesis employs a new strategy for starter unit generation.Organic letters · 2014Article
- A ketosynthase homolog uses malonyl units to form esters in cervimycin biosynthesis.Nature chemical biology · 2011Article
- Exploration of geosmin synthase from Streptomyces peucetius ATCC 27952 by deletion of doxorubicin biosynthetic gene cluster.Journal of industrial microbiology & biotechnology · 2009Article
- Biosynthesis of aromatic polyketides in bacteria.Accounts of chemical research · 2009Review
- Evolution of polyketide synthases in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Biosynthetic investigations of lactonamycin and lactonamycin z: cloning of the biosynthetic gene clusters and discovery of an unusual starter unit.Antimicrobial agents and chemotherapy · 2008Article
- Engineered biosynthesis of aklanonic acid analogues.Journal of the American Chemical Society · 2005Article
- Optimization of Streptomyces peucetius var. caesius N47 cultivation and epsilon-rhodomycinone production using experimental designs and response surface methods.Journal of industrial microbiology & biotechnology · 2004Article
- Engineering anthracycline biosynthesis toward angucyclines.Antimicrobial agents and chemotherapy · 2003Article
- Biosynthesis of polyketides in heterologous hosts.Microbiology and molecular biology reviews : MMBR · 2001Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The starter unit used in the biosynthesis of daunorubicin is propionyl coenzyme A (CoA) rather than acetyl-CoA, which is used in the production of most of the bacterial aromatic polyketides studied to date. In the daunorubicin biosynthesis gene cluster of Streptomyces peucetius, directly downstream of the genes encoding the beta-ketoacyl:acyl carrier protein synthase subunits, are two genes, dpsC and dpsD, encoding proteins that are believed to function as the starter unit-specifying enzymes. Recombinant strains containing plasmids carrying dpsC and dpsD, in addition to other daunorubicin polyketide synthase (PKS) genes, incorporate the correct starter unit into polyketides made by these genes, suggesting that, contrary to earlier reports, the enzymes encoded by dpsC and dpsD play a crucial role in starter unit specification. Additionally, the results of a cell-free synthesis of 21-carbon polyketides from propionyl-CoA and malonyl-CoA that used the protein extracts of recombinant strains carrying other daunorubicin PKS genes to which purified DpsC was added suggest that this enzyme has the primary role in starter unit discrimination for daunorubicin biosynthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.