ArticleJournal of bacteriology1999
Functional analysis of the carbohydrate-binding domains of Erwinia chrysanthemi Cel5 (Endoglucanase Z) and an Escherichia coli putative chitinase.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 54 citations in OpenAlex.
- Biochemical Characterization of Multimodular Xylanolytic Carbohydrate Esterases from the Marine Bacterium Flavimarina sp. Hel_I_48.Chembiochem : a European journal of chemical biology · 2025Article
- Exploring roles of the chitinase ChiC in modulatingMicrobiology spectrum · 2024Article
- Resources and Methods for Engineering "Designer" Glycan-Binding Proteins.Molecules (Basel, Switzerland) · 2021Review
- Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.Journal of molecular modeling · 2019Article
- Origin, evolution, and divergence of plant class C GH9 endoglucanases.BMC evolutionary biology · 2018Article
- The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.Plant molecular biology · 2017Article
- Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting.Acta naturae · 2014Article
- The first identification of carbohydrate binding modules specific to chitosan.The Journal of biological chemistry · 2013Article
- Structure and function of Pseudomonas aeruginosa protein PA1324 (21-170).Protein science : a publication of the Protein Society · 2009Article
- Sequence and structural features of carbohydrate binding in proteins and assessment of predictability using a neural network.BMC structural biology · 2007Article
- The modular architecture of Cellvibrio japonicus mannanases in glycoside hydrolase families 5 and 26 points to differences in their role in mannan degradation.The Biochemical journal · 2003Article
- Characterization of chitinase genes from an alkaliphilic actinomycete, Nocardiopsis prasina OPC-131.Applied and environmental microbiology · 2003Article
- Influence of deletions within domain II of exotoxin A on its extracellular secretion from Pseudomonas aeruginosa.Journal of bacteriology · 2000Article
- Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cel5 cellulase (formerly known as endoglucanase Z) from Erwinia chrysanthemi is a multidomain enzyme consisting of a catalytic domain, a linker region, and a cellulose binding domain (CBD). A three-dimensional structure of the CBD(Cel5) has previously been obtained by nuclear magnetic resonance. In order to define the role of individual residues in cellulose binding, site-directed mutagenesis was performed. The role of three aromatic residues (Trp18, Trp43, and Tyr44) in cellulose binding was demonstrated. The exposed potential hydrogen bond donors, residues Gln22 and Glu27, appeared not to play a role in cellulose binding, whereas residue Asp17 was found to be important for the stability of Cel5. A deletion mutant lacking the residues Asp17 to Pro23 bound only weakly to cellulose. The sequence of CBD(Cel5) exhibits homology to a series of five repeating domains of a putative large protein, referred to as Yheb, from Escherichia coli. One of the repeating domains (Yheb1), consisting of 67 amino acids, was cloned from the E. coli chromosome and purified by metal chelating chromatography. While CBD(Cel5) bound to both cellulose and chitin, Yheb1 bound well to chitin, but only very poorly to cellulose. The Yheb protein contains a region that exhibits sequence homology with the catalytic domain of a chitinase, which is consistent with the hypothesis that the Yheb protein is a chitinase.
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