ArticleJournal of bacteriology1999
Genetic characterization of a cell envelope-associated proteinase from Lactobacillus helveticus CNRZ32.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 86 citations in OpenAlex.
- Comparative analysis of proteolytic activity in lactic and propionic acid bacteria: substrate specificity and complementary effects in dairy fermentation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- The Enzyme Gene Expression of Protein Utilization and Metabolism byMicroorganisms · 2022Article
- Use of Mass Spectrometry to Profile Peptides in Whey Protein Isolate Medium Fermented byFrontiers in nutrition · 2019Article
- Production of Bioactive Peptides byFrontiers in microbiology · 2018Review
- The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions ofFrontiers in microbiology · 2017Article
- The naturally competent strain Streptococcus thermophilus LMD-9 as a new tool to anchor heterologous proteins on the cell surface.Microbial cell factories · 2014Article
- Lactobacillus helveticus: the proteolytic system.Frontiers in microbiology · 2013Article
- The fast milk acidifying phenotype of Streptococcus thermophilus can be acquired by natural transformation of the genomic island encoding the cell-envelope proteinase PrtS.Microbial cell factories · 2011Article
- Group-specific comparison of four lactobacilli isolated from human sources using differential blast analysis.Genes & nutrition · 2011Article
- Simultaneous presence of PrtH and PrtH2 proteinases in Lactobacillus helveticus Strains improves breakdown of the pure alphas1-casein.Applied and environmental microbiology · 2011Article
- prtH2, not prtH, is the ubiquitous cell wall proteinase gene in Lactobacillus helveticus.Applied and environmental microbiology · 2009Article
- Variation in specificity of the PrtP extracellular proteinases in Lactococcus lactis and Lactobacillus paracasei subsp. paracasei.Folia microbiologica · 2009Article
- Characterization of the pattern of alphas1- and beta-casein breakdown and release of a bioactive peptide by a cell envelope proteinase from Lactobacillus delbrueckii subsp. lactis CRL 581.Applied and environmental microbiology · 2008Article
- Comparative high-density microarray analysis of gene expression during growth of Lactobacillus helveticus in milk versus rich culture medium.Applied and environmental microbiology · 2007Article
- Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate Lactobacillus paracasei subsp. paracasei.Folia microbiologica · 2007Article
- Analysis of the presence of prtR proteinase gene in natural isolates of Lactobacillus rhamnosus.Folia microbiologica · 2006Article
- Identification of endopeptidase genes from the genomic sequence of Lactobacillus helveticus CNRZ32 and the role of these genes in hydrolysis of model bitter peptides.Applied and environmental microbiology · 2005Article
- The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Identification and genetic characterization of a novel proteinase, PrtR, from the human isolate Lactobacillus rhamnosus BGT10.Applied and environmental microbiology · 2003Article
- Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis.Applied and environmental microbiology · 2003Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cell envelope-associated proteinase gene (prtH) was identified in Lactobacillus helveticus CNRZ32. The prtH gene encodes a protein of 1,849 amino acids and with a predicted molecular mass of 204 kDa. The deduced amino acid sequence of the prtH product has significant identity (45%) to that of the lactococcal PrtP proteinases. Southern blot analysis indicates that prtH is not broadly distributed within L. helveticus. A prtH deletion mutant of CNRZ32 was constructed to evaluate the physiological role of PrtH. PrtH is not required for rapid growth or fast acid production in milk by CNRZ32. Cell surface proteinase activity and specificity were determined by hydrolysis of alpha(s1)-casein fragment 1-23 by whole cells. A comparison of CNRZ32 and its prtH deletion mutant indicates that CNRZ32 has at least two cell surface proteinases that differ in substrate specificity.
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