ArticleAnnals of the rheumatic diseases1999
Quantitative analysis of synovial membrane inflammation: a comparison between automated and conventional microscopic measurements.
Article in Annals of the rheumatic diseases, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Efficacy of sodium butyrate adjunct therapy in shigellosis: a randomized, double-blind, placebo-controlled clinical trial.BMC infectious diseases · 2012Trial
- B Cell Synovitis and Clinical Phenotypes in Rheumatoid Arthritis: Relationship to Disease Stages and Drug Exposure.Arthritis & rheumatology (Hoboken, N.J.) · 2020Article
- Potent Inducers of Endogenous Antimicrobial Peptides for Host Directed Therapy of Infections.Scientific reports · 2016Article
- Transdermal cannabidiol reduces inflammation and pain-related behaviours in a rat model of arthritis.European journal of pain (London, England) · 2016Article
- Ciprofloxacin Affects Host Cells by Suppressing Expression of the Endogenous Antimicrobial Peptides Cathelicidins and Beta-Defensin-3 in Colon Epithelia.Antibiotics (Basel, Switzerland) · 2014Article
- Article
- Evaluation of arthroscopy and macroscopic scoring.Arthritis research & therapy · 2009Article
- Utility of synovial biopsy.Arthritis research & therapy · 2009Review
- Validation and use of a computer-assisted counting procedure to quantify BrdU-labeled proliferating cells in the early postnatal mouse hippocampus.Journal of neuroscience methods · 2008Article
- Computer-assisted validation of the synovitis score.Virchows Archiv : an international journal of pathology · 2008Article
- Microscopic measurement of inflammation in synovial tissue: inter-observer agreement for manual quantitative, semiquantitative and computerised digital image analysis.Annals of the rheumatic diseases · 2007Article
- Limited effects of high-dose intravenous immunoglobulin (IVIG) treatment on molecular expression in muscle tissue of patients with inflammatory myopathies.Annals of the rheumatic diseases · 2007Article
- Standardisation of synovial tissue infiltrate analysis: how far have we come? How much further do we need to go?Annals of the rheumatic diseases · 2006Review
- Synovial tissue inflammation in early and late osteoarthritis.Annals of the rheumatic diseases · 2005Article
- Cathepsin k is a critical protease in synovial fibroblast-mediated collagen degradation.The American journal of pathology · 2001Article
- Demonstration of mast cell chemotactic activity in synovial fluid from rheumatoid patients.Annals of the rheumatic diseases · 2001Article
- Dynamics of early synovial cytokine expression in rodent collagen-induced arthritis : a therapeutic study using a macrophage-deactivating compound.The American journal of pathology · 2001Article
- Oligo- and monoarticular inflammatory arthritis: a need and a hope.Current rheumatology reports · 2000Review
- Synovial biopsy in arthritis research: five years of concerted European collaboration.Annals of the rheumatic diseases · 2000Review
- Interindividual and intra-articular variation of proinflammatory cytokines in patients with rheumatoid arthritis: potential implications for treatment.Annals of the rheumatic diseases · 2000Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe objective of this study was to quantify selected features of chronic synovial tissue inflammation by computerised image analysis and to validate the results by comparison with conventional microscopic measurements.
methodsSynovial biopsy samples were obtained from the knee joints of patients with chronic arthritis and prepared for immunohistochemical analysis using standard techniques. Following the development of special software, four parameters of chronic synovial inflammation were evaluated: intimal layer thickness, CD3+ cell infiltration, CD8+ cell infiltration and vascularity. Intimal layer thickness was expressed in microns. The intensity of CD3+ and CD8+ cell infiltration was expressed as the percentage area of the tissue section occupied by positively stained cells. Vascularity was expressed as the percentage area occupied by blood vessels. Conventional quantitative microscopic analysis was also undertaken and the results from both methods compared.
resultsSeventy eight tissue sections were selected for study. Measurements of intimal layer thickness by both techniques correlated strongly: r = 0.85, p = 0.0006. Measurements of CD8+ cell infiltration, usually widely dispersed, also correlated well: r = 0.64, p = 0.005. Measurements of CD3+ cell infiltration, often densely aggregated, correlated less well: r = 0.55, p = 0.02. Measurements of vascularity demonstrated no statistically significant correlation: r = 0.41, p = 0.07. Proficiency in the use of computerised image analysis was readily acquired.
conclusionComputerised image analysis was successfully applied to the measurement of some features of synovial tissue inflammation. Further software development is required to validate measurement of blood vessels of variable size.
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