ArticleInfection and immunity1999
Characterization of a Haemophilus ducreyi mutant deficient in expression of cytolethal distending toxin.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Impact of CDT Toxin on Human Diseases.Toxins · 2016Review
- The Cytolethal Distending Toxin Contributes to Microbial Virulence and Disease Pathogenesis by Acting As a Tri-Perditious Toxin.Frontiers in cellular and infection microbiology · 2016Review
- Article
- Haemophilus ducreyi infection induces activation of the NLRP3 inflammasome in nonpolarized but not in polarized human macrophages.Infection and immunity · 2013Article
- A new functional site W115 in CdtA is critical for Aggregatibacter actinomycetemcomitans cytolethal distending toxin.PloS one · 2013Article
- Article
- Cytolethal distending toxin: a conserved bacterial genotoxin that blocks cell cycle progression, leading to apoptosis of a broad range of mammalian cell lineages.Microbiology (Reading, England) · 2011Review
- Identification of Haemophilus ducreyi genes expressed during human infection.Microbiology (Reading, England) · 2008Article
- Haemophilus ducreyi partially activates human myeloid dendritic cells.Infection and immunity · 2007Article
- Role of intrachain disulfides in the activities of the CdtA and CdtC subunits of the cytolethal distending toxin of Actinobacillus actinomycetemcomitans.Infection and immunity · 2006Article
- Resistance of human periodontal ligament fibroblasts to the cytolethal distending toxin of Actinobacillus actinomycetemcomitans.Journal of periodontology · 2005Article
- Cytolethal distending toxin is essential for Helicobacter hepaticus colonization in outbred Swiss Webster mice.Infection and immunity · 2005Article
- Transferable antibiotic resistance elements in Haemophilus influenzae share a common evolutionary origin with a diverse family of syntenic genomic islands.Journal of bacteriology · 2004Article
- In vitro and in vivo characterization of Helicobacter hepaticus cytolethal distending toxin mutants.Infection and immunity · 2004Article
- Haemophilus ducreyi requires an intact flp gene cluster for virulence in humans.Infection and immunity · 2003Article
- A CdtA-CdtC complex can block killing of HeLa cells by Haemophilus ducreyi cytolethal distending toxin.Infection and immunity · 2003Article
- Expression of the cytolethal distending toxin in a geographically diverse collection of Haemophilus ducreyi clinical isolates.Sexually transmitted infections · 2003Article
- The cytolethal distending toxin of Haemophilus ducreyi inhibits endothelial cell proliferation.Infection and immunity · 2002Article
- Immunopathogenesis of Haemophilus ducreyi infection (chancroid).Infection and immunity · 2002Review
- Oral bacterial disease and the science of cellular conversation.Journal of the Royal Society of Medicine · 2002Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Haemophilus ducreyi expresses a soluble cytolethal distending toxin (CDT) that kills HeLa, HEp-2, and other human epithelial cells in vitro. H. ducreyi CDT activity is encoded by a three-gene cluster (cdtABC), and antibody to the cdtC gene product can neutralize CDT activity in vitro (L. D. Cope, S. R. Lumbley, J. L. Latimer, J. Klesney-Tait, M. K. Stevens, L. S. Johnson, M. Purven, R. S. Munson, Jr., T. Lagergard, J. D. Radolf, and E. J. Hansen, Proc. Natl. Acad. Sci. USA 94:4056-4061, 1997). Culture supernatant fluid from a recombinant Escherichia coli strain containing the H. ducreyi cdtABC gene cluster readily killed both HeLa cells and HaCaT keratinocytes and had a modest inhibitory effect on the growth of human foreskin fibroblasts. Insertional inactivation of the cdtC gene in this recombinant E. coli strain eliminated the ability of this strain to kill HeLa cells and HaCaT keratinocytes. This mutated H. ducreyi cdtABC gene cluster was used to construct an isogenic H. ducreyi cdtC mutant. Monoclonal antibodies against the H. ducreyi CdtA, CdtB, and CdtC proteins were used to characterize protein expression by this cdtC mutant. Culture supernatant fluid from this H. ducreyi cdtC mutant did not detectably affect any of the human cells used in this study. The presence of the wild-type H. ducreyi cdtC gene in trans in this H. ducreyi mutant restored its ability to express a CDT that killed both HeLa cells and HaCaT keratinocytes. The isogenic H. ducreyi cdtC mutant was shown to be as virulent as its wild-type parent strain in the temperature-dependent rabbit model for experimental chancroid. Lack of expression of the H. ducreyi CdtC protein also did not affect the ability of this H. ducreyi mutant to survive in the skin of rabbits.
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