ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Roles of rapsyn and agrin in interaction of postsynaptic proteins with acetylcholine receptors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Who cites it
37 citing papers in PubMed, 93 citations in OpenAlex.
- Cytoplasmic region of beta-dystroglycan is essential for postsynaptic maturation and neuromuscular function in mice.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Maturation of a postsynaptic domain: Role of small Rho GTPases in organising nicotinic acetylcholine receptor aggregates at the vertebrate neuromuscular junction.Journal of anatomy · 2022Review
- Membraneless condensates by Rapsn phase separation as a platform for neuromuscular junction formation.Neuron · 2021Article
- The role of the dystrophin glycoprotein complex on the neuromuscular system.Neuroscience letters · 2020Review
- Engineered agrin attenuates the severity of experimental autoimmune myasthenia gravis.Muscle & nerve · 2018Article
- The mechanism of acetylcholine receptor in binding MuSK in myasthenia gravis and the role of HSP90 molecular chaperone.American journal of translational research · 2016Article
- Failure of lysosome clustering and positioning in the juxtanuclear region in cells deficient in rapsyn.Journal of cell science · 2015Article
- Muscle-specific kinase (MuSK) autoantibodies suppress the MuSK pathway and ACh receptor retention at the mouse neuromuscular junction.The Journal of physiology · 2014Article
- Alterations of cAMP-dependent signaling in dystrophic skeletal muscle.Frontiers in physiology · 2013Review
- Acetylcholine receptor organization in membrane domains in muscle cells: evidence for rapsyn-independent and rapsyn-dependent mechanisms.The Journal of biological chemistry · 2011Article
- Patient autoantibodies deplete postsynaptic muscle-specific kinase leading to disassembly of the ACh receptor scaffold and myasthenia gravis in mice.The Journal of physiology · 2010Article
- Statistical analysis of high-resolution light microscope images reveals effects of cytoskeleton-disrupting drugs on the membrane organization of the nicotinic acetylcholine receptor.The Journal of membrane biology · 2010Article
- The roles of the dystrophin-associated glycoprotein complex at the synapse.Molecular neurobiology · 2010Review
- alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clustering.Molecular brain · 2008Article
- HSP90 beta regulates rapsyn turnover and subsequent AChR cluster formation and maintenance.Neuron · 2008Article
- The function of Shp2 tyrosine phosphatase in the dispersal of acetylcholine receptor clusters.BMC neuroscience · 2008Article
- Rapsyn carboxyl terminal domains mediate muscle specific kinase-induced phosphorylation of the muscle acetylcholine receptor.Neuroscience · 2008Article
- Muscle-like nicotinic receptor accessory molecules in sensory hair cells of the inner ear.Molecular and cellular neurosciences · 2008Article
- Dp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures.Neuroreport · 2007Article
- Beta-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
At the neuromuscular junction, aggregates of acetylcholine receptors (AChRs) are anchored in the muscle membrane by association with rapsyn and other postsynaptic proteins. We have investigated the interactions between the AChR and these proteins in cultured C2 myotubes before and after treatment with agrin, a nerve-derived protein that induces AChRs to cluster. When AChRs were isolated from detergent extracts of untreated C2 myotubes, they were associated with rapsyn and, to a lesser degree, with utrophin, beta-dystroglycan, MuSK, and src-related kinases, but not with syntrophin. Treatment with agrin increased the association of AChRs with MuSK, a receptor tyrosine kinase that forms part of the agrin receptor complex, without affecting other interactions. Analysis of rapsyn-deficient myotubes, which do not form protein clusters in response to agrin, revealed that rapsyn is required for association of the AChR with utrophin and beta-dystroglycan, and for the agrin-induced increase in association with MuSK, but not for constitutive interactions with MuSK and src-related kinases. In rapsyn -/- myotubes, agrin caused normal tyrosine phosphorylation of AChR-associated and total MuSK, whereas phosphorylation of the AChR beta subunit, both constitutive and agrin-induced, was strongly reduced. These results show first that aneural myotubes contain preassembled AChR protein complexes that may function in the assembly of the postsynaptic apparatus, and second that rapsyn, in addition to its role in AChR phosphorylation, mediates selected protein interactions with the AChR and serves as a link between the AChR and the dystrophin/utrophin glycoprotein complex.
Indexed as
Identifiers
10414969PMC6782834W1811859149What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.