ArticlePlant molecular biology1999
Characterization, subcellular localization and nuclear targeting of casein kinase 2 from Zea mays.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- Co-Localization of Insulin-Like Growth Factor Binding Protein-1, Casein Kinase-2β, and Mechanistic Target of Rapamycin in Human Hepatocellular Carcinoma Cells as Demonstrated by Dual Immunofluorescence and in Situ Proximity Ligation Assay.The American journal of pathology · 2018Article
- Specific characteristics of CK2β regulatory subunits in plants.Molecular and cellular biochemistry · 2011Article
- Role of plant-specific N-terminal domain of maize CK2β1 subunit in CK2β functions and holoenzyme regulation.PloS one · 2011Article
- Phosphorylation of maize eukaryotic translation initiation factor 5A (eIF5A) by casein kinase 2: identification of phosphorylated residue and influence on intracellular localization of eIF5A.The Journal of biological chemistry · 2010Article
- Yeast holoenzyme of protein kinase CK2 requires both beta and beta' regulatory subunits for its activity.Molecular and cellular biochemistry · 2007Article
- Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 alpha-subunit genes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Protein kinase CK2 modulates developmental functions of the abscisic acid responsive protein Rab17 from maize.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Cloning and characterization of the cDNA coding for the catalytic alpha subunit of CK2 from tobacco.Molecular and cellular biochemistry · 2001Article
- Distinctive features of plant protein kinase CK2.Molecular and cellular biochemistry · 2001Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. The sequence of the exons is 100% homologous to the sequence of the alphaCK2-1 cDNA. Southern hybridization of total genomic DNA from maize embryos with aCK2 cDNA indicated that the alphaCK2-1 gene is part of a multigenic family. We also isolated a new embryo cDNA clone coding for an alphaCK2-2 subunit. We studied the regulation of the enzyme in embryos at the mRNA level, at the protein level and by activity testing. By using immunocytochemistry the CK2 protein was localized in several types of cells of mature embryos. Particularly strong signals were visible in the cytoplasm of epidermis and meristematic cells. Decoration of nuclei of root cortex and scutellum cells was also observed suggesting that CK2 can shift from the cytoplasm into nuclei in specific cell types. We examined whether CK2 contained specific protein domains which actively target the protein to the nucleus by using in-frame fusions of the maize CK2alpha subunit to the reporter gene encoding beta-glucuronidase (GUS) which were assayed in transiently transformed onion epidermal cells. Analysis of chimeric constructs identified one region containing a nuclear localization signal (NLS) that is highly conserved in other alphaCK2 proteins.
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