ArticleJournal of muscle research and cell motility1999
Analysis of myosin heavy chains at the protein level in horse skeletal muscle.
Article in Journal of muscle research and cell motility, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 95 citations in OpenAlex.
- Profiling the epigenomic landscape of late embryonic and adult mouse hind limb muscles.Scientific reports · 2026Article
- Developmental, physiologic and phylogenetic perspectives on the expression and regulation of myosin heavy chains in mammalian skeletal muscles.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Review
- Short Communication: Supplementation with calcium butyrate causes an increase in the percentage of oxidative fibers in equine gluteus medius muscle.Journal of animal science · 2022Article
- Skeletal Muscle Adaptations to Exercise Training in Young and Aged Horses.Frontiers in aging · 2021Article
- Immune Response in Young Thoroughbred Racehorses under Training.Animals : an open access journal from MDPI · 2020Article
- The New Frontier in Oxytocin Physiology: The Oxytonic Contraction.International journal of molecular sciences · 2020Review
- Oxytocin/Osteocalcin/IL-6 and NGF/BDNF mRNA Levels in Response to Cold Stress Challenge in Mice: Possible Oxytonic Brain-Bone-Muscle-Interaction.Frontiers in physiology · 2019Article
- Skeletal muscle mitochondrial bioenergetics and associations with myostatin genotypes in the Thoroughbred horse.PloS one · 2017Article
- Functional Electrical Stimulation as a Safe and Effective Treatment for Equine Epaxial Muscle Spasms: Clinical Evaluations and Histochemical Morphometry of Mitochondria in Muscle Biopsies.European journal of translational myology · 2015Article
- Insights into the skeletal muscle characteristics of three southern African antelope species.Biology open · 2014Article
- Expression of MyHC genes, composition of muscle fiber type and their association with intramuscular fat, tenderness in skeletal muscle of Simmental hybrids.Molecular biology reports · 2014Article
- Muscle fibre type distribution of the thoracolumbar and hindlimb regions of horses: relating fibre type and functional role.Acta veterinaria Scandinavica · 2014Article
- Expression of the myosin heavy chain IIB gene in porcine skeletal muscle: the role of the CArG-Box promoter response element.PloS one · 2014Article
- Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama.Journal of anatomy · 2012Article
- Contractile properties of muscle fibers from the deep and superficial digital flexors of horses.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Immunohistochemical analysis of laryngeal muscles in normal horses and horses with subclinical recurrent laryngeal neuropathy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- A comprehensive characterisation of the fibre composition and properties of a limb (flexor digitorum superficialis, membri thoraci) and a trunk (psoas major) muscle in cattle.BMC cell biology · 2008Article
- Immunohistochemical analysis of myosin heavy chain expression in laryngeal muscles of the rabbit, cat, and baboon.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2008Article
- Myosin heavy chain profile of equine gluteus medius muscle following prolonged draught-exercise training and detraining.Journal of muscle research and cell motility · 2000Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Combined methodologies of enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting, traditional myofibrillar ATPase (mATPase) histochemistry and immunocytochemistry of whole biopsied samples were used to study myosin heavy chain (MHC) isoforms in the equine gluteus medius muscle. The ELISA technique allowed the quantification of the three MHC isoforms known to be present in different horse muscles: slow (MHC-I) and two fast (termed MHC-IIA and MCH-IIX). The SDS-PAGE method resolved MHCs in three bands: MHC-I, MHC-IIX and MHC-IIA from the fastest to the slowest migrating band and a quantification by densitometry for each MHC isoform was also possible. The identity of these three MHCs was confirmed by immunoblots with specific monoclonal antibodies. Five fibre types were defined immunohistochemically according to their MHC content: I, I + IIA, IIA, the hybrid IIAX and IIX. When quantitative data obtained with the four different methodologies were combined and compared, they were consistent and, when considered together, showed significant correlation. Nevertheless, the percentage of MHC-IIA histochemically derived was underestimated, while that of MHC-IIX was overestimated in comparison with the immunocytochemical determination of these MHC isoforms. The percentage of MHC-I obtained by ELISA technique was underestimated. In short, these integrated methods for the analysis of MHCs at the protein level demonstrate that equine skeletal muscle does not express the MHC-IIB, so type II fibres have been misclassified in numerous previous studies based upon the vary traditional mATPase histochemistry. They also offer new prospects for muscle fibre typing in equine experimental studies and veterinary medicine.
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