ArticleProceedings of the National Academy of Sciences of the United States of America1999
"Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 102 citations in OpenAlex.
- FgCot1 Regulates Polarized Growth and Conidiation in Fusarium graminearum via Gpmk1 MAPK and Tsf1 Transcriptional Pathways.Molecular plant pathology · 2026Article
- Programmable fluorescent aptamer-based RNA switches for rapid identification of point mutations.Nature chemistry · 2025Article
- NDR1/2 kinases regulate cell polarization and cell motility through Cdc42 GTPase and Pard3 signaling in mammalian cells.bioRxiv : the preprint server for biology · 2025Article
- The tissue proteome in the multi-omic landscape of kidney disease.Nature reviews. Nephrology · 2021Review
- Aptamers and their potential to selectively target aspects of EGF, Wnt/β-catenin and TGFβ-smad family signaling.International journal of molecular sciences · 2013Review
- Alterations in the Smad pathway in human cancers.Frontiers in bioscience (Landmark edition) · 2012Review
- Pneumocystis carinii interactions with lung epithelial cells and matrix proteins induce expression and activity of the PcSte20 kinase with subsequent phosphorylation of the downstream cell wall biosynthesis kinase PcCbk1.Infection and immunity · 2011Article
- Aptamers against prion proteins and prions.Cellular and molecular life sciences : CMLS · 2009Review
- [Monoclonal antibodies. Principles, generation, application, and side effects].Der Internist · 2008Review
- The eleven-year switch of peptide aptamers.Journal of biology · 2008Review
- Smad4 cooperates with lymphoid enhancer-binding factor 1/T cell-specific factor to increase c-myc expression in the absence of TGF-beta signaling.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Selective inhibition of TGF-beta responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP.Oncogene · 2005Article
- Intracellular expression of Peptide fusions for demonstration of protein essentiality in bacteria.Antimicrobial agents and chemotherapy · 2003Article
- The role of adaptor protein Ste50-dependent regulation of the MAPKKK Ste11 in multiple signalling pathways of yeast.Current genetics · 2003Review
- Genetic selection for modulators of a retinoic-acid-responsive reporter in human cells.Genetics · 2003Article
- Article
- Cyclin D3 activates Caspase 2, connecting cell proliferation with cell death.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genetic synthetic lethality screen at the single gene level in cultured human cells.Nucleic acids research · 2001Article
- Characterization and use of green fluorescent proteins from Renilla mulleri and Ptilosarcus guernyi for the human cell display of functional peptides.Journal of protein chemistry · 2001Article
- The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We selected peptide aptamers from combinatorial libraries that disrupted cell-cycle arrest caused by mating pheromone in yeast. We used these aptamers as baits in two-hybrid hunts to identify genes involved in cell-cycle arrest. These experiments identified genes known to function in the pathway, as well as a protein kinase, the CBK1 product, whose function was not known. We used a modified two-hybrid system to identify specific interactions disrupted by these aptamers. These experiments demonstrate a means to perform "genetics" on the protein complement of a cell without altering its genetic material. Peptide aptamers can be identified that disrupt a process. These aptamers can then be used as affinity reagents to identify individual proteins and protein interactions needed for the process. Forward genetic analysis with peptide aptamer "mutagens" should be particularly useful in elucidating genetic networks in organisms and processes for which classical genetics is not feasible.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.