ArticleProceedings of the National Academy of Sciences of the United States of America1999
Gene silencing via protein-mediated subcellular localization of DNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 38 citations in OpenAlex.
- Insights into ParB spreading from the complex structure of Spo0J and parS.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- How to build segregation complexes in bacteria: Use bridges.Genes & development · 2014Article
- Single-molecule analysis of proteinxDNA complexes formed during partition of newly replicated plasmid molecules in Streptococcus pyogenes.The Journal of biological chemistry · 2009Article
- VirB alleviates H-NS repression of the icsP promoter in Shigella flexneri from sites more than one kilobase upstream of the transcription start site.Journal of bacteriology · 2009Article
- Effects of the P1 plasmid centromere on expression of P1 partition genes.Journal of bacteriology · 2002Article
- Identification of the partitioning site within the repABC-type replicon of the composite Paracoccus versutus plasmid pTAV1.Journal of bacteriology · 2001Article
- Intracellular localization of P1 ParB protein depends on ParA and parS.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- On the mechanism of silencing in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
We previously reported that overexpression of SopB, an Escherichia coli F plasmid-encoded partition protein, led to silencing of genes linked to, but well-separated from, a cluster of SopB-binding sites termed sopC. We show here that in this SopB-mediated repression of sopC-linked genes, all but the N-terminal 82 amino acids of SopB can be replaced by the DNA-binding domain of a sequence-specific DNA-binding protein, provided that the sopC locus is also replaced by the recognition sequence of the DNA-binding domain. These results, together with our previous finding that the N-terminal fragment of SopB is responsible for its polar localization in cells, suggest a mechanism of gene silencing: patches of closely packed DNA-binding domains are formed if a sequence-specific DNA-binding protein is localized to specific cellular sites; such a patch can capture a DNA carrying the recognition site of the DNA-binding domain and sequestrate genes adjacent to the recognition site through nonspecific binding of DNA. The generalization of this model to gene silencing in eukaryotes is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.