ArticleProceedings of the National Academy of Sciences of the United States of America1999
A model for the mechanism of strand passage by DNA gyrase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Arabidopsis epigenetic factor AS2 mitigates nucleolar stress and promotes flat leaf development by repressing a CDK inhibitor.Development (Cambridge, England) · 2026Article
- Key Proteins and Enzymatic Mechanisms Underlying Drug Resistance in Mycobacterium tuberculosis.Advances in experimental medicine and biology · 2026Review
- Inhibition of DNA cleavage and strand passage activities of Mycobacterium tuberculosis topoisomerase I.Nucleic acids research · 2025Article
- Different propensities for gate opening in gyrases and topoisomerase IV.Nucleic acids research · 2025Article
- Structural basis of chiral wrap and T-segment capture byProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structural insights into the assembly of type IIA topoisomerase DNA cleavage-religation center.Nucleic acids research · 2024Article
- Structures of African swine fever virus topoisomerase complex and their implications.Nature communications · 2024Article
- Article
- Differential cellular localization of DNA gyrase and topoisomerase IB in response to DNA damage in Deinococcus radiodurans.Extremophiles : life under extreme conditions · 2023Article
- Rapid Detection of Quinolone Resistance Mutations inPathogens (Basel, Switzerland) · 2022Article
- Modulated control of DNA supercoiling balance by the DNA-wrapping domain of bacterial gyrase.Nucleic acids research · 2020Article
- Article
- CryoEM structures of open dimers of gyrase A in complex with DNA illuminate mechanism of strand passage.eLife · 2018Article
- Gyrase containing a single C-terminal domain catalyzes negative supercoiling of DNA by decreasing the linking number in steps of two.Nucleic acids research · 2018Article
- Topoisomerases as anticancer targets.The Biochemical journal · 2018Review
- Activities of gyrase and topoisomerase IV on positively supercoiled DNA.Nucleic acids research · 2017Article
- Thiophene antibacterials that allosterically stabilize DNA-cleavage complexes with DNA gyrase.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structural Dynamics and Mechanochemical Coupling in DNA Gyrase.Journal of molecular biology · 2016Article
- DNA Topoisomerases.EcoSal Plus · 2015Review
- Plasmid-mediated quinolone resistance.Microbiology spectrum · 2014Review
2 more citing papers are in PubMed but not listed here.
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3 authors.
Funding
Abstract
The mechanism of type II DNA topoisomerases involves the formation of an enzyme-operated gate in one double-stranded DNA segment and the passage of another segment through this gate. DNA gyrase is the only type II topoisomerase able to introduce negative supercoils into DNA, a feature that requires the enzyme to dictate the directionality of strand passage. Although it is known that this is a consequence of the characteristic wrapping of DNA by gyrase, the detailed mechanism by which the transported DNA segment is captured and directed through the DNA gate is largely unknown. We have addressed this mechanism by probing the topology of the bound DNA segment at distinct steps of the catalytic cycle. We propose a model in which gyrase captures a contiguous DNA segment with high probability, irrespective of the superhelical density of the DNA substrate, setting up an equilibrium of the transported segment across the DNA gate. The overall efficiency of strand passage is determined by the position of this equilibrium, which depends on the superhelical density of the DNA substrate. This mechanism is concerted, in that capture of the transported segment by the ATP-operated clamp induces opening of the DNA gate, which in turn stimulates ATP hydrolysis.
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