ArticleRNA (New York, N.Y.)1999
Influence of specific mutations on the thermal stability of the td group I intron in vitro and on its splicing efficiency in vivo: a comparative study.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Characterization of group I introns in generating circular RNAs as vaccines.Nucleic acids research · 2025Article
- Article
- RNA molecules with conserved catalytic cores but variable peripheries fold along unique energetically optimized pathways.RNA (New York, N.Y.) · 2011Article
- Toward predicting self-splicing and protein-facilitated splicing of group I introns.RNA (New York, N.Y.) · 2008Article
- Real-time control of the energy landscape by force directs the folding of RNA molecules.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- RNA chaperone activity of L1 ribosomal proteins: phylogenetic conservation and splicing inhibition.Nucleic acids research · 2007Article
- RNA chaperone activity and RNA-binding properties of the E. coli protein StpA.Nucleic acids research · 2007Article
- Self-splicing of a group I intron reveals partitioning of native and misfolded RNA populations in yeast.RNA (New York, N.Y.) · 2006Article
- Stabilities of HIV-1 DIS type RNA loop-loop interactions in vitro and in vivo.Nucleic acids research · 2006Article
- Influence of RNA structural stability on the RNA chaperone activity of the Escherichia coli protein StpA.Nucleic acids research · 2005Article
- Intracellular folding of the Tetrahymena group I intron depends on exon sequence and promoter choice.RNA (New York, N.Y.) · 2004Article
- Mutagenesis of a light-regulated psbA intron reveals the importance of efficient splicing for photosynthetic growth.Nucleic acids research · 2003Article
- Monitoring intermediate folding states of the td group I intron in vivo.The EMBO journal · 2002Article
- RNA chaperone StpA loosens interactions of the tertiary structure in the td group I intron in vivo.Genes & development · 2002Article
- RNA folding in living cells.RNA biologyReview
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group I introns constitute excellent systems for analyzing the relationship between RNA tertiary folding and catalysis. Within a hierarchical framework interpretation of RNA folding, secondary structure motifs subtend RNA three-dimensional (3D) architecture. Thus, mutations in two-dimensional motifs are expected to have effects different from those disrupting 3D contacts. Using UV spectroscopy, we have studied the influence of nucleotide substitutions, in both secondary and tertiary structure elements, on the thermal stability of the tertiary folding of the bacteriophage T4 td group I intron. Further, we present a quantitative analysis of the relationship between the splicing efficiency in vivo and the stability of the intron structure as monitored by UV melting curves. We conclude that the stability of the tertiary structure of a group I intron as measured by UV melting is generally a good indication of its ability to splice in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.