ArticleRNA (New York, N.Y.)1999
SR proteins are required for nematode trans-splicing in vitro.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans.FEBS letters · 2023Article
- Finding exonic islands in a sea of non-coding sequence: splicing related constraints on protein composition and evolution are common in intron-rich genomes.Genome biology · 2008Article
- A novel SR-related protein is required for the second step of Pre-mRNA splicing.Molecular and cellular biology · 2005Article
- Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome.Molecular and cellular biology · 2004Article
- Advances in the study of SR protein family.Genomics, proteomics & bioinformatics · 2003Review
- Multiple roles for SR proteins in trans splicing.Molecular and cellular biology · 2002Article
- Regulation of SR protein localization during development.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Functional selection of splicing enhancers that stimulate trans-splicing in vitro.RNA (New York, N.Y.) · 2001Article
- Functional characterization of SR and SR-related genes in Caenorhabditis elegans.The EMBO journal · 2000Article
- Developmental regulation of SR protein phosphorylation and activity.Genes & development · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
SR (ser/arg) proteins have been shown to play roles in numerous aspects of pre-mRNA splicing, including modulation of alternative splicing, commitment of substrates to the splicing pathway, and splice site communication. The last of these, splice site communication, is particularly relevant to trans-splicing in which the 5' and 3' exons originate on separate molecules. The participation of SR proteins in naturally occurring, spliced leader RNA-dependent transsplicing has not been examined. Here, we have isolated SR proteins from an organism that performs both trans- and cis-splicing, the nematode Ascaris lumbricoides. To examine their activity in in vitro splicing reactions, we have also developed and characterized an SR protein-depleted whole-cell extract. When tested in this extract, the nematode SR proteins are required for both trans- and cis-splicing. In addition, the state of phosphorylation of the nematode SR proteins is critical to their activity in vitro. Interestingly, mammalian (HeLa) and A. lumbricoides SR proteins exhibit equivalent activities in cis-splicing, while the nematode SR proteins are much more active in trans-splicing. Thus, it appears that SR proteins purified from an organism that naturally trans-splices its pre-mRNAs promote this reaction to a greater extent than do their mammalian counterparts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.