Evidence map›Paper›PMID 10411129›Full record

ArticleRNA (New York, N.Y.)1999

Positions in the 30S ribosomal subunit proximal to the 790 loop as determined by phenanthroline cleavage.

G W Muth, S P Hennelly, W E Hill

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

G W MuthDepartment of Chemistry, The University of Montana, Missoula 59812, USA.
S P Hennelly
W E Hill
University of Montana · US

Funding

PROBING RIBOSOMAL FUNCTIONR01GM035717 · NIGMS · UNIVERSITY OF MONTANA · PI HILL, WALTER E · 1986 to 2003
$1.3M
NIGMS NIH HHS NIGMS 35717
6 · The paper itself

Abstract

Positioning rRNA within the ribosome remains a challenging problem. Such positioning is critical to understanding ribosome function, as various rRNA regions interact to form suitable binding sites for ligands, such as tRNA and mRNA. We have used phenanthroline, a chemical nuclease, as a proximity probe, to help elucidate the regions of rRNA that are near neighbors of the stem-loop structure centering at nt 790 in the 16S rRNA of the Escherichia coli 30S ribosomal subunit. Using phenanthroline covalently attached to a DNA oligomer complementary to nt 787-795, we found that nt 582-584, 693-694, 787-790, and 795-797 were cleaved robustly and must lie within about 15 A of the tethered site at the 5' end of the DNA oligomer, which is adjacent to nt 795 of 16S rRNA.

Indexed as

Nucleic Acid ConformationBase SequenceDNA PrimersEscherichia coliModels, MolecularPhenanthrolinesRibosomesRNA ProbesRNA, Ribosomal, 16SDNA PrimersPhenanthrolinesRNA ProbesRNA, Ribosomal, 16S

Identifiers

PMID10411129
PMCPMC1369810
OpenAlexW2150927135

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.