ArticleMolecular and cellular biology1999
Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 164 citations in OpenAlex.
- The molecular basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2026Review
- BRD4: From molecular understanding to therapeutics development.Molecular cell · 2026Review
- Identification of human RNA Polymerase II interactors at early stages of transcription.bioRxiv : the preprint server for biology · 2025Article
- DNA lesion bypass and the stochastic dynamics of transcription-coupled repair.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Live-cell imaging uncovers the relationship between histone acetylation, transcription initiation, and nucleosome mobility.Science advances · 2023Article
- Bayesian inference of polymerase dynamics over the exclusion process.Royal Society open science · 2023Article
- Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patterns.The EMBO journal · 2023Article
- Discovery of a large-scale, cell-state-responsive allosteric switch in the 7SK RNA using DANCE-MaP.Molecular cell · 2022Article
- Phosphorylated viral protein evades plant immunity through interfering the function of RNA-binding protein.PLoS pathogens · 2022Article
- Live imaging of transcription sites using an elongating RNA polymerase II-specific probe.The Journal of cell biology · 2022Article
- Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.Molecular cell · 2021Article
- Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene.Nature communications · 2021Article
- The C4 protein encoded by tomato leaf curl Yunnan virus reverses transcriptional gene silencing by interacting with NbDRM2 and impairing its DNA-binding ability.PLoS pathogens · 2020Article
- Transcription-driven genome organization: a model for chromosome structure and the regulation of gene expression tested through simulations.Nucleic acids research · 2018Review
- Quantifying Single mRNA Translation Kinetics in Living Cells.Cold Spring Harbor perspectives in biology · 2018Review
- Imaging of native transcription factors and histone phosphorylation at high resolution in live cells.The Journal of cell biology · 2018Article
- Coactivators and general transcription factors have two distinct dynamic populations dependent on transcription.The EMBO journal · 2017Article
- Exploiting native forces to capture chromosome conformation in mammalian cell nuclei.Molecular systems biology · 2016Article
- Isolation of the protein and RNA content of active sites of transcription from mammalian cells.Nature protocols · 2016Article
- RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis.The Plant cell · 2016Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Various complexes that contain the core subunits of RNA polymerase II associated with different transcription factors have been isolated from eukaryotes; their precise molecular constitution depends on the purification procedure. We estimated the numbers of various components of such complexes in an HeLa cell by quantitative immunoblotting. The cells were lysed with saponin in a physiological buffer; approximately 140,000 unengaged polymerases (mainly of form IIA) were released. Only approximately 4,000 of these soluble molecules sedimented in glycerol gradients as holoenzyme-sized complexes. About 180,000 molecules of polymerases (approximately 110,000 molecules of form IIO) and 10,000 to 30,000 molecules of each of TFIIB, TFIIEalpha, TFIIEbeta, TFIIF-RAP74, TFIIF-RAP30, and TFIIH-MAT1 remained tightly associated with the nuclear substructure. Most proteins and run-on activity were retained when approximately 50% of the chromatin was detached with a nuclease, but approximately 45,000 molecules of bound TATA binding protein (TBP) were detached. Similar results were obtained after cross-linking living cells with formaldehyde. The results provide little support for the existence of a large pool of soluble holoenzyme; they are consistent with TBP-promoter complexes in nuclease-sensitive chromatin being assembled into preinitiation complexes attached to the underlying structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.