ArticleMolecular and cellular biology1999
Mutations in elongation factor 1beta, a guanine nucleotide exchange factor, enhance translational fidelity.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 72 citations in OpenAlex.
- Mutational analysis reveals potential phosphorylation sites in eukaryotic elongation factor 1A that are important for its activity.FEBS letters · 2021Article
- Demonstration of translation elongation factor 3 activity from a non-fungal species, Phytophthora infestans.PloS one · 2018Article
- Review
- The rate of metabolism as a factor determining longevity of the Saccharomyces cerevisiae yeast.Age (Dordrecht, Netherlands) · 2016Article
- The C-terminal region of human eukaryotic elongation factor 1Bδ.Journal of biomolecular NMR · 2016Article
- Genome-wide functional analysis of Plasmodium protein phosphatases reveals key regulators of parasite development and differentiation.Cell host & microbe · 2014Article
- Mutations in the chromodomain-like insertion of translation elongation factor 3 compromise protein synthesis through reduced ATPase activity.The Journal of biological chemistry · 2014Article
- Article
- Hydroxyapatite-coated sillicone rubber enhanced cell adhesion and it may be through the interaction of EF1β and γ-actin.PloS one · 2014Article
- Review
- Translation initiation requires cell division cycle 123 (Cdc123) to facilitate biogenesis of the eukaryotic initiation factor 2 (eIF2).The Journal of biological chemistry · 2013Article
- T-2 toxin induced Salmonella Typhimurium intoxication results in decreased Salmonella numbers in the cecum contents of pigs, despite marked effects on Salmonella-host cell interactions.Veterinary research · 2012Article
- Translational connection of TGFβ signaling: Phosphorylation of eEF1A1 by TβR-I inhibits protein synthesis.Small GTPases · 2011Article
- The eukaryotic translation elongation Factor 1Bgamma has a non-guanine nucleotide exchange factor role in protein metabolism.The Journal of biological chemistry · 2010Article
- Fast transcriptional responses to domestication in the brook charr Salvelinus fontinalis.Genetics · 2010Article
- Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast.BMC molecular biology · 2009Article
- Coordination of eukaryotic translation elongation factor 1A (eEF1A) function in actin organization and translation elongation by the guanine nucleotide exchange factor eEF1Balpha.The Journal of biological chemistry · 2009Article
- Guanine nucleotide exchange factor independence of the G-protein eEF1A through novel mutant forms and biochemical properties.The Journal of biological chemistry · 2008Article
- Article
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Translation elongation factor 1beta (EF-1beta) is a member of the family of guanine nucleotide exchange factors, proteins whose activities are important for the regulation of G proteins critical to many cellular processes. EF-1beta is a highly conserved protein that catalyzes the exchange of bound GDP for GTP on EF-1alpha, a required step to ensure continued protein synthesis. In this work, we demonstrate that the highly conserved C-terminal region of Saccharomyces cerevisiae EF-1beta is sufficient for normal cell growth. This region of yeast and metazoan EF-1beta and the metazoan EF-1beta-like protein EF-1delta is highly conserved. Human EF-1beta, but not human EF-1delta, is functional in place of yeast EF-1beta, even though both EF-1beta and EF-1delta have previously been shown to have guanine nucleotide exchange activity in vitro. Based on the sequence and functional homology, mutagenesis of two C-terminal residues identical in all EF-1beta protein sequences was performed, resulting in mutants with growth defects and sensitivity to translation inhibitors. These mutants also enhance translational fidelity at nonsense codons, which correlates with a reduction in total protein synthesis. These results indicate the critical function of EF-1beta in regulating EF-1alpha activity, cell growth, translation rates, and translational fidelity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.