ArticleJournal of clinical microbiology1999
PCR and blood culture for detection of Escherichia coli bacteremia in rats.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 103 citations in OpenAlex.
- Phenotypic and Molecular Study of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- First report of acute African swine fever in pig farms on the west coast of India: pathological and molecular insights.Frontiers in cellular and infection microbiology · 2025Article
- Persistence of commensal multidrug-resistantFrontiers in microbiology · 2024Article
- Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation.Environmental science and pollution research international · 2022Article
- Rapid DNA visual detection of polymicrobial bloodstream infection using filter paper.Scientific reports · 2022Article
- Infection Responsive Smart Delivery of Antibiotics Using Recombinant Spider Silk Nanospheres.Pharmaceutics · 2021Article
- Transmission of Similar Mcr-1 Carrying Plasmids among DifferentAntibiotics (Basel, Switzerland) · 2021Article
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- Foraging at Solid Urban Waste Disposal Sites as Risk Factor for Cephalosporin and Colistin ResistantFrontiers in microbiology · 2020Article
- Article
- Piperacillin enhances the inhibitory effect of tazobactam on β-lactamase through inhibition of organic anion transporter 1/3 in rats.Asian journal of pharmaceutical sciences · 2019Article
- Clonal diversity, virulence genes content and subclone status of Escherichia coli sequence type 131: comparative analysis of E. coli ST131 and non-ST131 isolates from Iran.BMC microbiology · 2019Article
- Article
- Study of class 1 integrons in multidrug-resistant uropathogenicIranian journal of basic medical sciences · 2018Article
- Molecular characterization of antibiotic resistantSaudi journal of biological sciences · 2018Article
- Poultry hatcheries as potential reservoirs for antimicrobial-resistant Escherichia coli: A risk to public health and food safety.Scientific reports · 2018Article
- Cytotoxic Escherichia coli strains encoding colibactin colonize laboratory mice.Microbes and infection · 2016Article
- Wild Birds, Frequent Carriers of Extended-Spectrum β-Lactamase (ESBL) Producing Escherichia coli of CTX-M and SHV-12 Types.Microbial ecology · 2016Article
- Phenotypic and genotypic analysis of pathogenicSaudi journal of biological sciences · 2016Article
- Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection.Scientific reports · 2015Article
Corrections and comments
- Commented on by
- Commented on by
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Critically ill patients often develop symptoms of sepsis and therefore require microbiological tests for bacteremia that use conventional blood culture (BC) techniques. However, since these patients frequently receive early empirical antibiotic therapy before diagnostic procedures are completed, examination by BC can return false-negative results. We therefore hypothesized that PCR could improve the rate of detection of microbial pathogens over that of BC. To test this hypothesis, male Wistar rats were challenged intravenously with 10(6) CFU of Escherichia coli. Blood was then taken at several time points for detection of E. coli by BC and by PCR with E. coli-specific primers derived from the uidA gene, encoding beta-glucuronidase. In further experiments, cefotaxime (100 or 50 mg/kg of body weight) was administered intravenously to rats 10 min after E. coli challenge. Without this chemotherapy, the E. coli detection rate decreased at 15 min and at 210 min after challenge from 100% to 62% of the animals with PCR and from 100% to 54% of the animals with BC (P, >0.05). Chemotherapy decreased the E. coli detection rate at 25 min and at 55 min after challenge from 100% to 50% with PCR and from 100% to 0% with BC (P, <0.05). Thus, at clinically relevant serum antibiotic levels, PCR affords a significantly higher detection rate than BC in this rat model. The results suggest that PCR could be a useful adjunct tool supplementing conventional BC techniques in diagnosing bacteremia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.