ArticleJournal of clinical microbiology1999
Specific detection of fusarium species in blood and tissues by a PCR technique.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 105 citations in OpenAlex.
- A Seminested PCR Method for the Diagnosis of Invasive Fungal Infections in Combat Injured.Open forum infectious diseases · 2024Article
- Clinical Features and Outcomes of Invasive Fusariosis: A Case Series in a Single Center with Literature Review.Infection & chemotherapy · 2023Article
- Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia.Biomedica : revista del Instituto Nacional de Salud · 2022Article
- Molecular Detection of Filamentous Fungi in Formalin-Fixed Paraffin-Embedded Specimens in Invasive Fungal Wound Infections Is Feasible with High Specificity.Journal of clinical microbiology · 2019Article
- Detection ofJournal of fungi (Basel, Switzerland) · 2019Article
- Rhizopus arrhizus and Fusarium solani Concomitant Infection in an Immunocompromised Host.Mycopathologia · 2016Article
- Molecular Diagnosis of Pathogenic Sporothrix Species.PLoS neglected tropical diseases · 2015Article
- Fusarium solani infection in a kidney transplant recipient.Indian journal of nephrology · 2014Article
- Review
- Fusarium falciforme vertebral abscess and osteomyelitis: case report and molecular classification.Journal of clinical microbiology · 2011Article
- Identification of Fusarium species in formalin-fixed and paraffin-embedded sections by in situ hybridization using peptide nucleic acid probes.Journal of clinical microbiology · 2011Article
- Fusarium infection in lung transplant patients: report of 6 cases and review of the literature.Medicine · 2011Review
- Fusarium solani: An Emerging Fungus in Chronic Diabetic Ulcer.Journal of laboratory physicians · 2010Article
- Detection of Fusarium tricinctum from cereal grain using PCR assay.Journal of applied genetics · 2008Article
- Fusarium infections in immunocompromised patients.Clinical microbiology reviews · 2007Review
- Localized cutaneous hyalohyphomycosis caused by a Fusarium species infection in a renal transplant patient.Journal of clinical microbiology · 2003Article
- Analysis of phylogenetic relationship of Cylindrocarpon lichenicola and Acremonium falciforme to the Fusarium solani species complex and a review of similarities in the spectrum of opportunistic infections caused by these fungi.Journal of clinical microbiology · 2002Article
- Study of the role of antimicrobial glucosinolate-derived isothiocyanates in resistance of Arabidopsis to microbial pathogens.Plant physiology · 2001Article
- Rapid detection and identification of Candida, Aspergillus, and Fusarium species in ocular samples using nested PCR.Journal of clinical microbiology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusarium species are opportunistic nosocomial pathogens that often cause fatal invasive mycoses. We designed a primer pair that amplifies by PCR a fragment of a gene coding for the rRNA of Fusarium species. The DNAs of the main Fusarium species and Neocosmospora vasinfecta but not the DNAs from 11 medically important fungi were amplified by these primers. The lower limit of detection of the PCR system was 10 fg of Fusarium solani DNA by ethidium bromide staining. To test the ability of this PCR system to detect Fusarium DNA in tissues, we developed a mouse model of disseminated fusariosis. Using the PCR, we detected Fusarium DNA in mouse tissues and in spiked human blood. Furthermore, F. solani, Fusarium moniliforme, and Fusarium oxysporum were testing by random amplified polymorphic DNA (RAPD) analysis. The bands produced by RAPD analysis were purified, cloned, and sequenced. The information was used to design primer pairs that selectively amplified one or several Fusarium species. The method developed may be useful for the rapid detection and identification of Fusarium species both from culture and from clinical samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.