ArticleThe Journal of cell biology1999
Intracellular localization of proteasomal degradation of a viral antigen.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
77 citing papers in PubMed, 211 citations in OpenAlex.
- A CK2 and SUMO-dependent, PML NB-involved regulatory mechanism controlling BLM ubiquitination and G-quadruplex resolution.Nature communications · 2023Article
- Diagnostic performance of a novel antigen-capture ELISA for the detection of SARS-CoV-2.Analytical biochemistry · 2023Article
- Detection of SARS-CoV-2 by antigen ELISA test is highly swayed by viral load and sample storage condition.Expert review of anti-infective therapy · 2022Article
- A few good peptides: MHC class I-based cancer immunosurveillance and immunoevasion.Nature reviews. Immunology · 2021Review
- How multi-component cascades operate in cells: lessons from the ubiquitin system-containing liquid-separated condensates.Molecular & cellular oncology · 2021Article
- Batf3-Dependent Dendritic Cells Promote Optimal CD8 T Cell Responses against Respiratory Poxvirus Infection.Journal of virology · 2018Article
- Insufficient Innate Immunity Contributes to the Susceptibility of the Castaneous Mouse to Orthopoxvirus Infection.Journal of virology · 2017Article
- Microtubule Regulation and Function during Virus Infection.Journal of virology · 2017Review
- Varied Role of Ubiquitylation in Generating MHC Class I Peptide Ligands.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Caveolin-1 is an aggresome-inducing protein.Scientific reports · 2016Article
- I've got algorithm: predicting tumor and autoimmune peptide targets for CD8+ T cells.The Journal of clinical investigation · 2016Article
- The benefits of local depletion: The centrosome as a scaffold for ubiquitin-proteasome-mediated degradation.Cell cycle (Georgetown, Tex.) · 2016Review
- Ubiquitous Autofragmentation of Fluorescent Proteins Creates Abundant Defective Ribosomal Products (DRiPs) for Immunosurveillance.The Journal of biological chemistry · 2015Article
- PA28αβ: the enigmatic magic ring of the proteasome?Biomolecules · 2014Review
- MyD88-dependent immunity to a natural model of vaccinia virus infection does not involve Toll-like receptor 2.Journal of virology · 2014Article
- Identification and comparative analysis of hepatitis C virus-host cell protein interactions.Molecular bioSystems · 2013Article
- Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 is regulated by proteasome activity in tumour cells.PloS one · 2013Article
- Endogenous viral antigen processing generates peptide-specific MHC class I cell-surface clusters.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- MHC class I antigen processing distinguishes endogenous antigens based on their translation from cellular vs. viral mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The potential link between PML NBs and ICP0 in regulating lytic and latent infection of HSV-1.Protein & cell · 2012Review
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
To better understand proteasomal degradation of nuclear proteins and viral antigens we studied mutated forms of influenza virus nucleoprotein (NP) that misfold and are rapidly degraded by proteasomes. In the presence of proteasome inhibitors, mutated NP (dNP) accumulates in highly insoluble ubiquitinated and nonubiquitinated species in nuclear substructures known as promyelocytic leukemia oncogenic domains (PODs) and the microtubule organizing center (MTOC). Immunofluorescence revealed that dNP recruits proteasomes and a selective assortment of molecular chaperones to both locales, and that a similar (though less dramatic) effect is induced by proteasome inhibitors in the absence of dNP expression. Biochemical evidence is consistent with the idea that dNP is delivered to PODs/MTOC in the absence of proteasome inhibitors. Restoring proteasome activity while blocking protein synthesis results in disappearance of dNP from PODs and the MTOC and the generation of a major histocompatibility complex class I-bound peptide derived from dNP but not NP. These findings demonstrate that PODs and the MTOC serve as sites of proteasomal degradation of misfolded dNP and probably cellular proteins as well, and imply that antigenic peptides are generated at one or both of these sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.