ArticleThe Journal of cell biology1999
A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 175 citations in OpenAlex.
- The Diverse Cellular Functions of Inner Nuclear Membrane Proteins.Cold Spring Harbor perspectives in biology · 2021Review
- Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.Science advances · 2020Article
- Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress.eLife · 2019Article
- Antibody-Induced Internalization of HIV-1 Env Proteins Limits Surface Expression of the Closed Conformation of Env.Journal of virology · 2019Article
- MISTIC-fusion proteins as antigens for high quality membrane protein antibodies.Scientific reports · 2017Article
- Review
- Colonization of the Intestinal Tract of the Polyphagous Pest Spodoptera littoralis with the GFP-Tagged Indigenous Gut Bacterium Enterococcus mundtii.Frontiers in microbiology · 2016Article
- mRNA encoding Sec61β, a tail-anchored protein, is localized on the endoplasmic reticulum.Journal of cell science · 2015Article
- Nuclear membrane diversity: underlying tissue-specific pathologies in disease?Current opinion in cell biology · 2015Review
- Structure-based design of combinatorial mutagenesis libraries.Protein science : a publication of the Protein Society · 2015Article
- Actin network disassembly powers dissemination of Listeria monocytogenes.Journal of cell science · 2014Article
- Dynamic recruitment of protein tyrosine phosphatase PTPD1 to EGF stimulation sites potentiates EGFR activation.PloS one · 2014Article
- The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility.The Journal of cell biology · 2013Article
- A component of the Sec61 ER protein transporting pore is required for plant susceptibility to powdery mildew.Frontiers in plant science · 2013Article
- Many mechanisms, one entrance: membrane protein translocation into the nucleus.Cellular and molecular life sciences : CMLS · 2012Review
- Science stories: flies, planes, worms, and lasers.Molecular biology of the cell · 2011Article
- Evolution: functional evolution of nuclear structure.The Journal of cell biology · 2011Review
- Article
- System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics.The Journal of cell biology · 2011Article
- High-level fluorescence labeling of gram-positive pathogens.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear envelope (NE) is a distinct subdomain of the ER, but few membrane components have been described that are specific to it. We performed a visual screen in tissue culture cells to identify proteins targeted to the NE. This approach does not require assumptions about the nature of the association with the NE or the physical separation of NE and ER. We confirmed that screening a library of fusions to the green fluorescent protein can be used to identify proteins targeted to various subcompartments of mammalian cells, including the NE. With this approach, we identified a new NE membrane protein, named nurim. Nurim is a multispanning membrane protein without large hydrophilic domains that is very tightly associated with the nucleus. Unlike the known NE membrane proteins, it is neither associated with nuclear pores, nor targeted like lamin-associated membrane proteins. Thus, nurim is a new type of NE membrane protein that is localized to the NE by a distinct mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.