ArticleJournal of virology1999
Specific interaction between the hepatitis C virus NS5B RNA polymerase and the 3' end of the viral RNA.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Hijacking the cytoskeleton: association of HCV polymerase with α/β-tubulin and its potential relation to the viral replication and cell proliferation.Molecular and cellular biochemistry · 2026Article
- Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis.International journal of molecular sciences · 2026Article
- Ribosome Pausing at Inefficient Codons at the End of the Replicase Coding Region Is Important for Hepatitis C Virus Genome Replication.International journal of molecular sciences · 2020Article
- Hepatitis C Virus Translation Regulation.International journal of molecular sciences · 2020Review
- The Role of the RNA-RNA Interactome in the Hepatitis C Virus Life Cycle.International journal of molecular sciences · 2020Review
- RNA binding protein 24 regulates the translation and replication of hepatitis C virus.Protein & cell · 2018Article
- Identification of a Conserved RNA-dependent RNA Polymerase (RdRp)-RNA Interface Required for Flaviviral Replication.The Journal of biological chemistry · 2016Article
- The Coding Region of the HCV Genome Contains a Network of Regulatory RNA Structures.Molecular cell · 2016Article
- Inhibition of HCV translation by disrupting the structure and interactions of the viral CRE and 3' X-tail.Nucleic acids research · 2015Article
- Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase.Journal of virology · 2013Article
- Hepatitis C virus RNA: molecular switches mediated by long-range RNA-RNA interactions?Nucleic acids research · 2013Article
- A twist in the tail: SHAPE mapping of long-range interactions and structural rearrangements of RNA elements involved in HCV replication.Nucleic acids research · 2012Article
- Let-7b is a novel regulator of hepatitis C virus replication.Cellular and molecular life sciences : CMLS · 2012Article
- A Chinese Herbal Decoction, Modified Yi Guan Jian, Induces Apoptosis in Hepatic Stellate Cells through an ROS-Mediated Mitochondrial/Caspase Pathway.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Hepatitis C virus, human immunodeficiency virus and Pseudomonas phage PS5 triad share epitopes of immunogenic determinants.Virology journal · 2010Article
- All three domains of the hepatitis C virus nonstructural NS5A protein contribute to RNA binding.Journal of virology · 2010Article
- Hepatitis C virus NS5A protein down-regulates the expression of spindle gene Aspm through PKR-p38 signaling pathway.The Journal of biological chemistry · 2008Article
- A hepatitis C virus cis-acting replication element forms a long-range RNA-RNA interaction with upstream RNA sequences in NS5B.Journal of virology · 2008Article
- Exploiting cis-acting replication elements to direct hepatitis C virus-dependent transgene expression.Journal of virology · 2005Article
- Structural characterization of the highly conserved 98-base sequence at the 3' end of HCV RNA genome and the complementary sequence located at the 5' end of the replicative viral strand.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatitis C virus (HCV) NS5B protein is the viral RNA-dependent RNA polymerase capable of directing RNA synthesis. In this study, an electrophoretic mobility shift assay demonstrated the interaction between a partially purified recombinant NS5B protein and a 3' viral genomic RNA with or without the conserved 98-nucleotide tail. The NS5B-RNA complexes were specifically competed away by the unlabeled homologous RNA but not by the viral 5' noncoding region and very poorly by the 3' conserved 98-nucleotide tail. A 3' coding region with conserved stem-loop structures rather than the 3' noncoding region of the HCV genome is critical for the specific binding of NS5B. Nevertheless, no direct interaction between the 3' coding region and the HCV NS5A protein was detected. Furthermore, two independent RNA-binding domains (RBDs) of NS5B were identified, RBD1, from amino acid residues 83 to 194, and RBD2, from residues 196 to 298. Interestingly, the conserved motifs of RNA-dependent RNA polymerase for putative RNA binding (220-DxxxxD-225) and template/primer position (282-S/TGxxxTxxxNS/T-292) are present in the RBD2. Nevertheless, the RNA-binding activity of RBD2 was abolished when it was linked to the carboxy-terminal half of the NS5B. These results provide some clues to understanding the initiation of HCV replication.
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