ArticleJournal of virology1999
An avian sarcoma/leukosis virus-based gene trap vector for mammalian cells.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines.BMC biotechnology · 2019Article
- Ubiquibodies, synthetic E3 ubiquitin ligases endowed with unnatural substrate specificity for targeted protein silencing.The Journal of biological chemistry · 2014Article
- Mass spectrometry-based proteomic approaches for discovery of HIV-host interactions.Future virology · 2014Article
- Independent and high-level dual-gene expression in adult stem-progenitor cells from a single lentiviral vector.Gene therapy · 2009Article
- Design of an HIV-1 lentiviral-based gene-trap vector to detect developmentally regulated genes in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Identification of key residues in subgroup A avian leukosis virus envelope determining receptor binding affinity and infectivity of cells expressing chicken or quail Tva receptor.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RCASBP-M2C is a retroviral vector derived from an avian sarcoma/leukosis virus which has been modified so that it uses the envelope gene from an amphotropic murine leukemia virus (E. V. Barsov and S. H. Hughes, J. Virol. 70:3922-3929, 1996). The vector replicates efficiently in avian cells and infects, but does not replicate in, mammalian cells. This makes the vector useful for gene delivery, mutagenesis, and other applications in mammalian systems. Here we describe the development of a derivative of RCASBP-M2C, pGT-GFP, that can be used in gene trap experiments in mammalian cells. The gene trap vector pGT-GFP contains a green fluorescent protein (GFP) reporter gene. Appropriate insertion of the vector into genes causes GFP expression; this facilitates the rapid enrichment and cloning of the trapped cells and provides an opportunity to select subpopulations of trapped cells based on the subcellular localization of GFP. With this vector, we have generated about 90 gene-trapped lines using D17 and NIH 3T3 cells. Five trapped NIH 3T3 lines were selected based on the distribution of GFP in cells. The cellular genes disrupted by viral integration have been identified in four of these lines by using a 5' rapid amplification of cDNA ends protocol.
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Registered trials
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