ArticleJournal of virology1999
Neural stem cells as engraftable packaging lines can mediate gene delivery to microglia: evidence from studying retroviral env-related neurodegeneration.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- The Evolution of Stem Cells, Disease Modeling, and Drug Discovery for Neurological Disorders.Stem cells and development · 2020Review
- Astrocyte Infection Is Required for Retrovirus-Induced Spongiform Neurodegeneration Despite Suppressed Viral Protein Expression.Frontiers in neuroscience · 2019Article
- Rebound from Inhibition: Self-Correction against Neurodegeneration?Journal of clinical & cellular immunology · 2017Article
- Ecotropic Murine Leukemia Virus Infection of Glial Progenitors Interferes with Oligodendrocyte Differentiation: Implications for Neurovirulence.Journal of virology · 2016Article
- Postinhibitory rebound neurons and networks are disrupted in retrovirus-induced spongiform neurodegeneration.Journal of neurophysiology · 2014Article
- Targeting β-secretase with RNAi in neural stem cells for Alzheimer's disease therapy.Neural regeneration research · 2013Article
- Implications and limitations of cellular reprogramming for psychiatric drug development.Experimental & molecular medicine · 2013Review
- Unique N-linked glycosylation of CasBrE Env influences its stability, processing, and viral infectivity but not its neurotoxicity.Journal of virology · 2013Article
- Identification of a neuronal gene expression signature: role of cell cycle arrest in murine neuronal differentiation in vitro.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Article
- Retrovirus-induced spongiform neurodegeneration is mediated by unique central nervous system viral targeting and expression of env alone.Journal of virology · 2011Article
- Misfolding of CasBrE SU is reversed by interactions with 4070A Env: implications for gammaretroviral neuropathogenesis.Retrovirology · 2010Article
- Applications of neural and mesenchymal stem cells in the treatment of gliomas.Expert review of anticancer therapy · 2009Review
- Gene expression profiling of microglia infected by a highly neurovirulent murine leukemia virus: implications for neuropathogenesis.Retrovirology · 2006Article
- Molecular imaging of gene therapy for cancer.Gene therapy · 2004Review
- MCP-1 and CCR2 contribute to non-lymphocyte-mediated brain disease induced by Fr98 polytropic retrovirus infection in mice: role for astrocytes in retroviral neuropathogenesis.Journal of virology · 2004Article
- Viral vectors for gene delivery to the nervous system.Nature reviews. Neuroscience · 2003Review
- Selective gene expression in brain microglia mediated via adeno-associated virus type 2 and type 5 vectors.Gene therapy · 2003Article
- Global gene and cell replacement strategies via stem cells.Gene therapy · 2002Review
- Neural stem cells display extensive tropism for pathology in adult brain: evidence from intracranial gliomas.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Establishment and properties of neural stem cell clones: plasticity in vitro and in vivo.Brain pathology (Zurich, Switzerland) · 1999Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The induction of spongiform myeloencephalopathy by murine leukemia viruses is mediated primarily by infection of central nervous system (CNS) microglia. In this regard, we have previously shown that CasBrE-induced disease requires late, rather than early, virus replication events in microglial cells (W. P. Lynch et al., J. Virol. 70:8896-8907, 1996). Furthermore, neurodegeneration requires the presence of unique sequences within the viral env gene. Thus, the neurodegeneration-inducing events could result from microglial expression of retroviral envelope protein alone or from the interaction of envelope protein with other viral structural proteins in the virus assembly and maturation process. To distinguish between these possible mechanisms of disease induction, we engineered the engraftable neural stem cell line C17-2 into packaging/producer cells in order to deliver the neurovirulent CasBrE env gene to endogenous CNS cells. This strategy resulted in significant CasBrE env expression within CNS microglia without the appearance of replication competent virus. CasBrE envelope expression within microglia was accompanied by increased expression of activation markers F4/80 and Mac-1 (CD11b) but failed to induce spongiform neurodegenerative changes. These results suggest that envelope expression alone within microglia is not sufficient to induce neurodegeneration. Rather, microglia-mediated disease appears to require neurovirulent Env protein interaction with other viral proteins during assembly or maturation. More broadly, the results presented here prove the efficacy of a novel method by which neural stem cell biology may be harnessed for genetically manipulating the CNS, not only for studying neurodegeneration but also as a paradigm for the disseminated distribution of retroviral vector-transduced genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.