ArticleJournal of virology1999
Initiation of genomic plus-strand RNA synthesis from DNA and RNA templates by a viral RNA-dependent RNA polymerase.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Enhanced recovery of terminal sequences of viral RNAs via fine-tuning of the high-throughput sequencing library preparation and evidence of a non-templated nucleotide addition at the 3' end of the minus strand in members of the genusThe Journal of general virology · 2026Article
- Characterizing multiple exogenous and endogenous small RNA populations in parallel with subfemtomolar sensitivity using a streptavidin gel-shift assay.RNA (New York, N.Y.) · 2009Article
- Non-DNA-templated addition of nucleotides to the 3' end of RNAs by the mitochondrial RNA polymerase of Physarum polycephalum.Molecular and cellular biology · 2008Article
- Interaction between Brome mosaic virus proteins and RNAs: effects on RNA replication, protein expression, and RNA stability.Journal of virology · 2005Article
- Structural and functional analysis of the cis-acting elements required for plus-strand RNA synthesis of Bamboo mosaic virus.Journal of virology · 2005Article
- The conserved, 5' termini of RNAs 1 and 2 of Tomato aspermy virus are dispensable for infection but affect virulence.Virus genes · 2005Article
- Mutual interference between genomic RNA replication and subgenomic mRNA transcription in brome mosaic virus.Journal of virology · 2005Article
- Replicase-binding sites on plus- and minus-strand brome mosaic virus RNAs and their roles in RNA replication in plant cells.Journal of virology · 2004Article
- Repression and derepression of minus-strand synthesis in a plus-strand RNA virus replicon.Journal of virology · 2004Article
- Template sequence near the initiation nucleotide can modulate brome mosaic virus RNA accumulation in plant protoplasts.Journal of virology · 2004Article
- Stable RNA structures can repress RNA synthesis in vitro by the brome mosaic virus replicase.RNA (New York, N.Y.) · 2003Article
- Autonomous role of 3'-terminal CCCA in directing transcription of RNAs by Qbeta replicase.Journal of virology · 2001Article
- The brome mosaic virus RNA3 intergenic replication enhancer folds to mimic a tRNA TpsiC-stem loop and is modified in vivo.RNA (New York, N.Y.) · 2001Article
- Completion of RNA synthesis by viral RNA replicases.Nucleic acids research · 2001Article
- Polymerization of nontemplate bases before transcription initiation at the 3' ends of templates by an RNA-dependent RNA polymerase: an activity involved in 3' end repair of viral RNAs.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Recognition of the core RNA promoter for minus-strand RNA synthesis by the replicases of Brome mosaic virus and Cucumber mosaic virus.Journal of virology · 2000Article
- Template nucleotide moieties required for de novo initiation of RNA synthesis by a recombinant viral RNA-dependent RNA polymerase.Journal of virology · 2000Article
- RNA sequence and secondary structural determinants in a minimal viral promoter that directs replicase recognition and initiation of genomic plus-strand RNA synthesis.Journal of molecular biology · 1999Article
- Use of DNA, RNA, and chimeric templates by a viral RNA-dependent RNA polymerase: evolutionary implications for the transition from the RNA to the DNA world.Journal of virology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to the synthesis of minus-strand genomic and plus-strand subgenomic RNAs, the requirements for brome mosaic virus (BMV) genomic plus-strand RNA synthesis in vitro have not been previously reported. Therefore, little is known about the biochemical requirements for directing genomic plus-strand synthesis. Using DNA templates to characterize the requirements for RNA-dependent RNA polymerase template recognition, we found that initiation from the 3' end of a template requires one nucleotide 3' of the initiation nucleotide. The addition of a nontemplated nucleotide at the 3' end of minus-strand BMV RNAs led to initiation of genomic plus-strand RNA in vitro. Genomic plus-strand initiation was specific since cucumber mosaic virus minus-strand RNA templates were unable to direct efficient synthesis under the same conditions. In addition, mutational analysis of the minus-strand template revealed that the -1 nontemplated nucleotide, along with the +1 cytidylate and +2 adenylate, is important for RNA-dependent RNA polymerase interaction. Furthermore, genomic plus-strand RNA synthesis is affected by sequences 5' of the initiation site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.