ArticleJournal of virology1999
Subcellular localization and rolling circle replication of peach latent mosaic viroid: hallmarks of group A viroids.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 85 citations in OpenAlex.
- Diversity and evolution of viroids and viroid-like agents with circular RNA genomes revealed by metatranscriptome mining.Nucleic acids research · 2025Article
- Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells.Virology journal · 2024Article
- Cellular roadmaps of viroid infection.Trends in microbiology · 2023Review
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host.RNA biology · 2019Article
- RNAi mediated inhibition of viroid infection in transgenic plants expressing viroid-specific small RNAs derived from various functional domains.Scientific reports · 2015Article
- Elucidation of the structures of all members of the Avsunviroidae family.Molecular plant pathology · 2014Article
- What has been happening with viroids?Virus genes · 2014Review
- A pathogenic non coding RNA that replicates and accumulates in chloroplasts traffics to this organelle through a nuclear-dependent step.Plant signaling & behavior · 2012Article
- Studies on subcellular compartmentalization of plant pathogenic noncoding RNAs give new insights into the intracellular RNA-traffic mechanisms.Plant physiology · 2012Article
- A novel ribozyme-based prophylaxis inhibits influenza A virus replication and protects from severe disease.PloS one · 2011Article
- In-depth sequencing of the siRNAs associated with peach latent mosaic viroid infection.BMC molecular biology · 2010Article
- Identification of proteins from prunus persica that interact with peach latent mosaic viroid.Journal of virology · 2009Article
- Article
- Catalytic diversity of extended hammerhead ribozymes.Biochemistry · 2008Article
- A viroid RNA with a specific structural motif inhibits chloroplast development.The Plant cell · 2007Article
- The fittest versus the flattest: experimental confirmation of the quasispecies effect with subviral pathogens.PLoS pathogens · 2006Article
- An element of the tertiary structure of Peach latent mosaic viroid RNA revealed by UV irradiation.Journal of virology · 2006Article
- A short double-stranded RNA motif of Peach latent mosaic viroid contains the initiation and the self-cleavage sites of both polarity strands.Journal of virology · 2005Article
- Identification of a peach latent mosaic viroid hairpin able to act as a Dicer-like substrate.Journal of virology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We characterized the peach latent mosaic viroid (PLMVd) replication intermediates that accumulate in infected peach leaves and determined the tissue and subcellular localization of the RNA species. Using in situ hybridization, we showed that PLMVd strands of both plus and minus polarities concentrate in the cells forming the palisade parenchyma. At the cellular level, PLMVd was found to accumulate predominantly in chloroplasts. Northern blot analyses demonstrated that PLMVd replicates via a symmetric mode involving the accumulation of both circular and linear monomeric strands of both polarities. No multimeric conformer was detected, indicating that both strands self-cleave efficiently via their hammerhead sequences. Dot blot hybridizations revealed that PLMVd strands of both polarities accumulate equally but that the relative concentrations vary by more than 50-fold between peach cultivars. Taken together these results establish two hallmarks for the classification of viroids. Group A viroids (e.g., PLMVd), which possess hammerhead structures, replicate in the chloroplasts via the symmetric mode. By contrast, group B viroids, which share a conserved central region, replicate in the nucleus via an asymmetric mechanism. This is an important difference between self-cleaving and non-self-cleaving viroids, and the implications for the evolutionary origin and replication are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.