Evidence map›Paper›PMID 10400604›Full record

ArticleJournal of bacteriology1999

Methanobacterium thermoautotrophicum RNA polymerase and transcription in vitro.

T J Darcy, W Hausner, D E Awery, A M Edwards, M Thomm, J N Reeve

Open access · bronzeAbstract read
In one paragraph

Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 64 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

T J DarcyDepartment of Microbiology, The Ohio State University, Columbus, Ohio 43210, USA.
W Hausner
D E Awery
A M Edwards
M Thomm
J N Reeve
Kiel University · DEThe Ohio State University · USUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum DeltaH has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or recombinant forms of the M. thermoautotrophicum TATA-binding protein and transcription factor TFB. Efforts to obtain transcription initiation from hydrogen-regulated methane gene promoters were, however, unsuccessful. Two previously unrecognized archaeal RNAP subunits have been identified, and complex formation by the M. thermoautotrophicum RNAP and TFB has been demonstrated.

Indexed as

Archaeal ProteinsGene Expression Regulation, ArchaealTranscription Factor TFIIBTranscription, GeneticAmino Acid SequenceCentrifugation, Density GradientDNA-Binding ProteinsDNA-Directed RNA PolymerasesElectrophoresis, Polyacrylamide GelHydrogenMethaneMethanobacteriumMolecular Sequence DataNuclear ProteinsPrecipitin TestsTATA-Box Binding ProteinArchaeal ProteinsDNA-Binding ProteinsDNA-Directed RNA PolymerasesHydrogenMethaneNuclear ProteinsTATA-Box Binding Proteintranscription factor B, ArchaeaTranscription FactorsTranscription Factor TFIIB

Identifiers

PMID10400604
PMCPMC93948
OpenAlexW1520930575

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.