ArticleJournal of bacteriology1999
Genetic and molecular analysis of cglB, a gene essential for single-cell gliding in Myxococcus xanthus.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 80 citations in OpenAlex.
- Discovery of PilU as a second type IV pilus retraction motor inJournal of bacteriology · 2026Article
- Predation in microbial communities: gradients of nutritive killing.Nature reviews. Microbiology · 2026Review
- Bottleneck size drives the evolution of cooperative traits in an aggregative multicellular myxobacterium.PLoS biology · 2026Article
- Genetic and environmental determinants of streaming and aggregation in Myxococcus xanthus.Scientific reports · 2025Article
- Article
- Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility.Science advances · 2023Article
- Mutation ofFrontiers in microbiology · 2022Article
- Hidden paths to endless forms most wonderful: Complexity of bacterial motility shapes diversification of latent phenotypes.BMC evolutionary biology · 2020Article
- Kin recognition and outer membrane exchange (OME) in myxobacteria.Current opinion in microbiology · 2020Review
- Kin discrimination and outer membrane exchange in Myxococcus xanthus: Experimental analysis of a natural population.PloS one · 2019Article
- Parallel emergence of negative epistasis across experimental lineages.Evolution; international journal of organic evolution · 2017Article
- Reversals and collisions optimize protein exchange in bacterial swarms.Physical review. E · 2017Article
- Evolution by flight and fight: diverse mechanisms of adaptation by actively motile microbes.The ISME journal · 2017Article
- Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior.The ISME journal · 2016Article
- How Myxobacteria Cooperate.Journal of molecular biology · 2015Review
- Contact- and Protein Transfer-Dependent Stimulation of Assembly of the Gliding Motility Machinery in Myxococcus xanthus.PLoS genetics · 2015Article
- A genetic screen in Myxococcus xanthus identifies mutants that uncouple outer membrane exchange from a downstream cellular response.Journal of bacteriology · 2014Article
- Transmission of a signal that synchronizes cell movements in swarms of Myxococcus xanthus.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bacterial social networks: structure and composition of Myxococcus xanthus outer membrane vesicle chains.Environmental microbiology · 2014Article
- Molecular recognition in myxobacterial outer membrane exchange: functional, social and evolutionary implications.Molecular microbiology · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gliding movements of individual isolated Myxococcus xanthus cells depend on the genes of the A-motility system (agl and cgl genes). Mutants carrying defects in those genes are unable to translocate as isolated cells on solid surfaces. The motility defect of cgl mutants can be transiently restored to wild type by extracellular complementation upon mixing mutant cells with wild-type or other motility mutant cells. To develop a molecular understanding of the function of a Cgl protein in gliding motility, we cloned the cglB wild-type allele by genetic complementation of the mutant phenotype. The nucleotide sequence of a 2.85-kb fragment was determined and shown to encode two complete open reading frames. The CglB protein was determined to be a 416-amino-acid putative lipoprotein with an unusually high cysteine content. The CglB antigen localized to the membrane fraction. The swarming and gliding defects of a constructed DeltacglB mutant were fully restored upon complementation with the cglB wild-type allele. Experiments with a cglB allele encoding a CglB protein with a polyhistidine tag at the C terminus showed that this allele also promoted wild-type levels of swarming and single-cell gliding, but was unable to stimulate DeltacglB cells to move. Possible functions of CglB as a mechanical component or as a signal protein in single cell gliding are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.