ArticleJournal of bacteriology1999
A novel Sinorhizobium meliloti operon encodes an alpha-glucosidase and a periplasmic-binding-protein-dependent transport system for alpha-glucosides.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Fruit residues as substrates for single-cell oil production by Rhodococcus species: physiology and genomics of carbohydrate catabolism.World journal of microbiology & biotechnology · 2024Article
- Article
- Finding approximate gene clusters with Gecko 3.Nucleic acids research · 2016Article
- MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula.Plant physiology · 2016Article
- Genetic redundancy is prevalent within the 6.7 Mb Sinorhizobium meliloti genome.Molecular genetics and genomics : MGG · 2015Article
- Examination of prokaryotic multipartite genome evolution through experimental genome reduction.PLoS genetics · 2014Article
- Carbohydrate catabolism in Phaeobacter inhibens DSM 17395, a member of the marine roseobacter clade.Applied and environmental microbiology · 2014Article
- Identification and characterization of a NaCl-responsive genetic locus involved in survival during desiccation in Sinorhizobium meliloti.Applied and environmental microbiology · 2013Article
- The thuEFGKAB operon of rhizobia and agrobacterium tumefaciens codes for transport of trehalose, maltitol, and isomers of sucrose and their assimilation through the formation of their 3-keto derivatives.Journal of bacteriology · 2013Article
- Role of trehalose in heat and desiccation tolerance in the soil bacterium Rhizobium etli.BMC microbiology · 2012Article
- Role of the Sinorhizobium meliloti global regulator Hfq in gene regulation and symbiosis.Molecular plant-microbe interactions : MPMI · 2010Article
- Responses of rhizobia to desiccation in relation to osmotic stress, oxygen, and temperature.Applied and environmental microbiology · 2007Review
- Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus.Journal of bacteriology · 2006Article
- sinI- and expR-dependent quorum sensing in Sinorhizobium meliloti.Journal of bacteriology · 2005Article
- The high-affinity maltose/trehalose ABC transporter in the extremely thermophilic bacterium Thermus thermophilus HB27 also recognizes sucrose and palatinose.Journal of bacteriology · 2005Article
- In silico and transcriptional analysis of carbohydrate uptake systems of Streptomyces coelicolor A3(2).Journal of bacteriology · 2004Article
- Redundancy in periplasmic binding protein-dependent transport systems for trehalose, sucrose, and maltose in Sinorhizobium meliloti.Journal of bacteriology · 2002Article
- Fructose uptake in Sinorhizobium meliloti is mediated by a high-affinity ATP-binding cassette transport system.Journal of bacteriology · 2001Article
- The Rhodobacter capsulatus genome.Photosynthesis research · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The most abundant carbon source transported into legume root nodules is photosynthetically produced sucrose, yet the importance of its metabolism by rhizobia in planta is not yet known. To identify genes involved in sucrose uptake and hydrolysis, we screened a Sinorhizobium meliloti genomic library and discovered a segment of S. meliloti DNA which allows Ralstonia eutropha to grow on the alpha-glucosides sucrose, maltose, and trehalose. Tn5 mutagenesis localized the required genes to a 6.8-kb region containing five open reading frames which were named agl, for alpha-glucoside utilization. Four of these (aglE, aglF, aglG, and aglK) appear to encode a periplasmic-binding-protein-dependent sugar transport system, and one (aglA) appears to encode an alpha-glucosidase with homology to family 13 of glycosyl hydrolases. Cosmid-borne agl genes permit uptake of radiolabeled sucrose into R. eutropha cells. Analysis of the properties of agl mutants suggests that S. meliloti possesses at least one additional alpha-glucosidase as well as a lower-affinity transport system for alpha-glucosides. It is possible that the Fix+ phenotype of agl mutants on alfalfa is due to these additional functions. Loci found by DNA sequencing to be adjacent to aglEFGAK include a probable regulatory gene (aglR), zwf and edd, which encode the first two enzymes of the Entner-Doudoroff pathway, pgl, which shows homology to a gene encoding a putative phosphogluconolactonase, and a novel Rhizobium-specific repeat element.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.