ArticlePlant physiology1999
Purification and characterization of the reconstitutively active citrate carrier from maize mitochondria.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Multiple roles played by the mitochondrial citrate carrier in cellular metabolism and physiology.Cellular and molecular life sciences : CMLS · 2022Review
- Molecular Mechanism of Citrate Efflux by the Mitochondrial Citrate Transporter CT in Filamentous FungusFrontiers in microbiology · 2021Article
- Characterization of In Vivo Function(s) of Members of the Plant Mitochondrial Carrier Family.Biomolecules · 2020Review
- Metabolic Roles of Plant Mitochondrial Carriers.Biomolecules · 2020Review
- Plant Mitochondrial Carriers: Molecular Gatekeepers That Help to Regulate Plant Central Carbon Metabolism.Plants (Basel, Switzerland) · 2020Review
- Cardiolipin, a critical determinant of mitochondrial carrier protein assembly and function.Biochimica et biophysica acta · 2009Review
- Citrate transporters play a critical role in aluminium-stimulated citrate efflux in rice bean (Vigna umbellata) roots.Annals of botany · 2006Article
- Isolated durum wheat and potato cell mitochondria oxidize externally added NADH mostly via the malate/oxaloacetate shuttle with a rate that depends on the carrier-mediated transport.Plant physiology · 2003Article
- Purification and characterization of the reconstitutively active adenine nucleotide carrier from mitochondria of Jerusalem artichoke (Helianthus tuberosus L.) tubers.Journal of bioenergetics and biomembranes · 2002Article
- Kinetics of light emission and oxygen consumption by bioluminescent bacteria.Journal of bioenergetics and biomembranes · 2001Article
- Pattern of aluminum-induced secretion of organic acids differs between rye and wheat.Plant physiology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecular mass of 31 kD. When reconstituted into liposomes, the citrate carrier catalyzed a pyridoxal 5'-P-sensitive citrate/citrate exchange. It was purified 224-fold with a recovery of 50% and a protein yield of 0.22% with respect to the mitochondrial extract. In the reconstituted system the purified citrate carrier catalyzed a first-order reaction of citrate/citrate (0.065 min-1) or citrate/malate exchange (0.075 min-1). Among the various substrates and inhibitors tested, the reconstituted protein transported citrate, cis-aconitate, isocitrate, L-malate, succinate, malonate, glutarate, alpha-ketoglutarate, oxaloacetate, and alpha-ketoadipate and was inhibited by pyridoxal 5'-P, phenylisothiocyanate, mersalyl, and p-hydroxymercuribenzoate (but not N-ethylmaleimide), 1,2, 3-benzentricarboxylate, benzylmalonate, and butylmalonate. The activation energy of the citrate/citrate exchange was 66.5 kJ/mol between 10 degrees C and 35 degrees C; the half-saturation constant (Km) for citrate was 0.65 +/- 0.05 mM and the maximal rate (Vmax) of the citrate/citrate exchange was 13.0 +/- 1.0 micromol min-1 mg-1 protein at 25 degrees C.
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