ArticleMolecular biology of the cell1999
A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 56 citations in OpenAlex.
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- Tracing putative trafficking of the glycolytic enzyme enolase via SNARE-driven unconventional secretion.Eukaryotic cell · 2012Article
- The yeast Batten disease orthologue Btn1 controls endosome-Golgi retrograde transport via SNARE assembly.The Journal of cell biology · 2011Article
- A genomic analysis of chronological longevity factors in budding yeast.Cell cycle (Georgetown, Tex.) · 2011Article
- Aggregation of α-synuclein in S. cerevisiae is associated with defects in endosomal trafficking and phospholipid biosynthesis.Journal of molecular neuroscience : MN · 2011Article
- Insulin-regulated trafficking of GLUT4 requires ubiquitination.Traffic (Copenhagen, Denmark) · 2010Article
- Trans-Golgi network and endosome dynamics connect ceramide homeostasis with regulation of the unfolded protein response and TOR signaling in yeast.Molecular biology of the cell · 2008Article
- Btn2, a Hook1 ortholog and potential Batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast.Molecular and cellular biology · 2007Article
- The Sec1p/Munc18 protein Vps45p binds its cognate SNARE proteins via two distinct modes.The Journal of cell biology · 2006Article
- AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism.The EMBO journal · 2005Article
- Structure-based functional analysis reveals a role for the SM protein Sly1p in retrograde transport to the endoplasmic reticulum.Molecular biology of the cell · 2005Article
- Early stages of the secretory pathway, but not endosomes, are required for Cvt vesicle and autophagosome assembly in Saccharomyces cerevisiae.Molecular biology of the cell · 2004Article
- i-SNAREs: inhibitory SNAREs that fine-tune the specificity of membrane fusion.The Journal of cell biology · 2004Article
- How Tlg2p/syntaxin 16 'snares' Vps45.The EMBO journal · 2002Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the syntaxin protein family participate in the docking-fusion step of several intracellular vesicular transport events. Tlg1p has been identified as a nonessential protein required for efficient endocytosis as well as the maintenance of normal levels of trans-Golgi network proteins. In this study we independently describe Tlg1p as an essential protein required for cell viability. Depletion of Tlg1p in vivo causes a defect in the transport of the vacuolar protein carboxypeptidase Y through the early Golgi. Temperature-sensitive (ts) mutants of Tlg1p also accumulate the endoplasmic reticulum/cis-Golgi form of carboxypeptidase Y at the nonpermissive temperature (38 degrees C) and exhibit underglycosylation of secreted invertase. Overexpression of Tlg1p complements the growth defect of vti1-11 at the nonpermissive temperature, whereas incomplete complementation was observed with vti1-1, further suggesting a role for Tlg1p in the Golgi apparatus. Overexpression of Sed5p decreases the viability of tlg1 ts mutants compared with wild-type cells, suggesting that tlg1 ts mutants are more susceptible to elevated levels of Sed5p. Tlg1p is able to bind His6-tagged Sec17p (yeast alpha-SNAP) in a dose-dependent manner and enters into a SNARE complex with Vti1p, Tlg2p, and Vps45p. Morphological analyses by electron microscopy reveal that cells depleted of Tlg1p or tlg1 ts mutants incubated at the restrictive temperature accumulate 40- to 50-nm vesicles and experience fragmentation of the vacuole.
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