ArticlePlant molecular biology1999
Characterization of the pea rDNA replication fork barrier: putative cis-acting and trans-acting factors.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Chromatin dynamics and RNA metabolism are double-edged swords for the maintenance of plant genome integrity.Nature plants · 2024Review
- Genome-wide analysis of DNA replication and DNA double-strand breaks using TrAEL-seq.PLoS biology · 2021Article
- Review
- Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants.The Plant journal : for cell and molecular biology · 2015Article
- A telomerase-independent component of telomere loss in chromatin assembly factor 1 mutants of Arabidopsis thaliana.Chromosoma · 2013Article
- Transcription-replication encounters, consequences and genomic instability.Nature structural & molecular biology · 2013Review
- Dysfunction of chromatin assembly factor 1 induces shortening of telomeres and loss of 45S rDNA in Arabidopsis thaliana.The Plant cell · 2010Article
- Article
- Replication fork stalling at natural impediments.Microbiology and molecular biology reviews : MMBR · 2007Review
- Molecular architecture of a eukaryotic DNA replication terminus-terminator protein complex.Molecular and cellular biology · 2006Article
- Mechanisms of transcription-replication collisions in bacteria.Molecular and cellular biology · 2005Article
- swi1- and swi3-dependent and independent replication fork arrest at the ribosomal DNA of Schizosaccharomyces pombe.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Transcription termination factor reb1p causes two replication fork barriers at its cognate sites in fission yeast ribosomal DNA in vivo.Molecular and cellular biology · 2004Article
- Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It was previously shown that in pea (Pisum sativum), rDNA repeats contain a polar replication fork barrier that blocks progression of the replication machinery moving in the direction opposite to transcription. This barrier maps in the untranscribed spacer close to the 3' end of the 25S gene. Very similar barriers are also found in the rDNA of yeast, Xenopus and mammalian cultured cells. This high conservation indicates that the rDNA barrier plays a relevant biological role. Progression of replication forks through the DNA sequence where the barrier maps in pea was investigated in plasmids replicating in Escherichia coli and Saccharomyces cerevisiae. No barrier was detected in these heterologous systems, indicating that the DNA sequence by itself was insufficient to block the replication machinery. Therefore, trans-acting factors were likely to be required. Taking advantage of the natural sequence heterogeneity in pea rDNA, we obtained evidence that a 27 bp imperfect tandem repeat is involved in the arrest of replication. Moreover, nuclear protein(s) specifically bound to this repeat suggesting that this DNA/protein complex is responsible for the polar arrest of replication forks.
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