ArticleThe American journal of pathology1999
Suppression of prostate carcinoma cell invasion by expression of antisense L-plastin gene.
Article in The American journal of pathology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Meta-analysis of oncogenic protein kinase Ciota signaling in lung adenocarcinoma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2009Pooled it
- Dual inhibition of ABCE1 and LCP1 by microRNA-96 results in an additive effect in breast cancer mouse model.Oncotarget · 2019Article
- Exosomal Release of L-Plastin by Breast Cancer Cells Facilitates Metastatic Bone Osteolysis.Translational oncology · 2019Article
- Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer.PloS one · 2018Article
- Article
- AP4 modulated by the PI3K/AKT pathway promotes prostate cancer proliferation and metastasis of prostate cancer via upregulating L-plastin.Cell death & disease · 2017Article
- miR-375 Regulates Invasion-Related Proteins Vimentin and L-Plastin.The American journal of pathology · 2017Article
- Evidence for Critical Role of Lymphocyte Cytosolic Protein 1 in Oral Cancer.Scientific reports · 2017Article
- Plastin family of actin-bundling proteins: its functions in leukocytes, neurons, intestines, and cancer.International journal of cell biology · 2012Article
- Protein kinase CK1alphaLS promotes vascular cell proliferation and intimal hyperplasia.The American journal of pathology · 2010Article
- Carcinogen-altered genes in rat esophagus positively modulated to normal levels of expression by both black raspberries and phenylethyl isothiocyanate.Cancer research · 2008Article
- Biology and therapeutic potential of cannabinoid CB2 receptor inverse agonists.British journal of pharmacology · 2008Review
- Isolation of a human gallbladder cancer cell clone with high invasive phenotype in vitro and metastatic potential in orthotopic model and inhibition of its invasiveness by heparanase antisense oligodeoxynucleotides.Clinical & experimental metastasis · 2007Article
- H-prune-nm23-H1 protein complex and correlation to pathways in cancer metastasis.Journal of bioenergetics and biomembranes · 2006Article
- Discovering functional relationships between RNA expression and chemotherapeutic susceptibility using relevance networks.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- A novel association between the human heat shock transcription factor 1 (HSF1) and prostate adenocarcinoma.The American journal of pathology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Based on the finding that gene expression for the actin-bundling protein L-plastin is inducible by androgen and that L-plastin is overexpressed in malignant epithelium of the prostate, we examined the functional consequences of L-plastin down-regulation in prostate carcinoma cell lines by both transfection and retroviral infection. We constructed retroviral vectors to express two different regions of the L-plastin gene, a 1713-bp 3'-coding portion and a 163-bp 5'-untranslated region, both in antisense orientation. Introduction of either constructs into prostate carcinoma cell lines, PC-3 and its isogenic but metastatic variant PC-3M cells, reduced the growth rates of both cell lines. In vitro invasion and motility of PC-3 and PC-3M cells were drastically suppressed (approximately 10-fold) by the expression of the antisense constructs. Evidence was obtained to indicate that L-plastin protein levels were indeed decreased by the antisense expression. The antisense construct for the 5'-untranslated region with the most unique sequence for the L-plastin gene was more effective in down-regulation efficiency compared with the larger antisense construct in the coding region, which maintains homology to other members of the plastin gene family. Cells infected with the 163-bp antisense virus, which were also tested in a nude mouse diaphragm invasion model, showed suppression of in vivo invasion of both PC-3 and PC-3M cells. These results suggested that overexpression of L-plastin might be functionally involved in prostate cancer invasion and metastasis, and raised the possibility that L-plastin gene-specific antisense delivery could potentially be a useful approach to interfere with prostate cancer progression in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.