ArticleThe Biochemical journal1999
Talin contains three similar vinculin-binding sites predicted to form an amphipathic helix.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 76 citations in OpenAlex.
- Talin-tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation.The Journal of cell biology · 2026Article
- Hyperglycemic environments directly compromise intestinal epithelial barrier function in an organoid model and hyaluronan (∼35 kDa) protects via a layilin dependent mechanism.Matrix biology : journal of the International Society for Matrix Biology · 2024Article
- The actin binding sites of talin have both distinct and complementary roles in cell-ECM adhesion.PLoS genetics · 2024Article
- Understanding the molecular basis of cardiomyopathy.American journal of physiology. Heart and circulatory physiology · 2022Review
- Drosophila vinculin is more harmful when hyperactive than absent, and can circumvent integrin to form adhesion complexes.Journal of cell science · 2016Article
- Spider Silk Peptide Is a Compact, Linear Nanospring Ideal for Intracellular Tension Sensing.Nano letters · 2016Article
- Vinculin phosphorylation at Tyr1065 regulates vinculin conformation and tension development in airway smooth muscle tissues.The Journal of biological chemistry · 2014Article
- Knockdown of cathepsin B and uPAR inhibits CD151 and α3β1 integrin-mediated cell adhesion and invasion in glioma.Molecular carcinogenesis · 2013Article
- Review
- The interaction of vinculin with actin.PLoS computational biology · 2013Article
- Activation of vinculin induced by cholinergic stimulation regulates contraction of tracheal smooth muscle tissue.The Journal of biological chemistry · 2011Article
- A molecular dynamics investigation of vinculin activation.Biophysical journal · 2010Article
- Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein.The Journal of biological chemistry · 2010Article
- Structural and biophysical properties of the integrin-associated cytoskeletal protein talin.Biophysical reviews · 2009Article
- Control of high affinity interactions in the talin C terminus: how talin domains coordinate protein dynamics in cell adhesions.The Journal of biological chemistry · 2009Article
- Structural determinants of integrin binding to the talin rod.The Journal of biological chemistry · 2009Article
- Vinculin binding in its closed conformation by a helix addition mechanism.The EMBO journal · 2007Article
- Structural dynamics of alpha-actinin-vinculin interactions.Molecular and cellular biology · 2005Article
- Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle.The EMBO journal · 2004Article
- Talin1 is critical for force-dependent reinforcement of initial integrin-cytoskeleton bonds but not tyrosine kinase activation.The Journal of cell biology · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using recombinant talin polypeptides and an SDS/PAGE-blot overlay assay, we have previously identified three regions of talin that are involved in binding to vinculin [Gilmore, Wood, Ohanian, Jackson, Patel, Rees, Hynes and Critchley (1993) J. Cell Biol. 122, 337-347]. We have confirmed these observations by using a yeast two-hybrid assay and shown that talin residues 498-656, 852-950 and 1929-2029 are each capable of binding to vinculin residues 1-258. We have further defined the three vinculin-binding sites in talin to residues 607-636, 852-876 and 1944-1969; alignment of these sequences shows 59% similarity, although there are only two identical residues. Predictions of secondary structure indicate that this vinculin-binding motif forms an amphipathic alpha-helix. The hydrophobic face of helix 607-636 contains three aligned leucines (residues 608, 615 and 622), which show conservative substitutions in the other two sites. To test the possibility that this might constitute a leucine zipper involved in vinculin binding, we mutated each leucine residue to an alanine. The results showed that this leucine repeat is not essential to the interaction between talin and vinculin. We also used the yeast two-hybrid system to define further the talin-binding site within vinculin residues 1-258. C-terminal deletions made in accordance with exon boundaries showed that vinculin residues 1-167 are capable of interacting with each of the three vinculin-binding sites in talin. However, all N-terminal deletions abolished binding. The results suggest that the talin-binding site in vinculin has a relatively complex fold, whereas the vinculin-binding motif in talin is contained within a short linear peptide sequence that is repeated three times in the talin rod domain.
Indexed as
Identifiers
10393080PMC1220354W2073187664What OpenQuestion holds
Registered trials
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