Evidence map›Paper›PMID 10391845›Full record

ArticleClinical and diagnostic laboratory immunology1999

Cytokine mRNA expression in lesions in cats with chronic gingivostomatitis.

R Harley, C R Helps, D A Harbour, T J Gruffydd-Jones, M J Day

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and diagnostic laboratory immunology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 77 citations in OpenAlex.

  1. Trial
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  15. Chronic Gingivostomatitis with Esophagitis in Cats.Journal of veterinary internal medicine · 2017
    Article
  16. Relationship betweenFrontiers in veterinary science · 2017
    Article
  17. Article
  18. Article
  19. Molecular diagnostic assays for infectious diseases in cats.The Veterinary clinics of North America. Small animal practice · 2010
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

R HarleyDepartment of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom. R.Harley@bris.ac.uk
C R Helps
D A Harbour
T J Gruffydd-Jones
M J Day
University of Bristol · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Semiquantitative reverse transcription-PCR assays were developed to measure feline interleukin-2 (IL-2), IL-4, IL-5, IL-6, IL-10, and IL-12 (p35 & p40); gamma interferon (IFN-gamma); and glyceraldehyde-3-phosphate dehydrogenase mRNA concentrations in biopsies of feline oral mucosa. Biopsies were collected from 30 cats with chronic gingivostomatitis (diseased) prior to each cat receiving one of four treatments. In 23 cases replicate biopsies were collected 3 months after treatment commenced. Biopsies were also analyzed from 11 cats without clinical disease (nondiseased). Expression of IL-2, IL-10, IL-12 (p35 and p40), and IFN-gamma was detected in most nondiseased biopsies, while IL-6 was detected in a minority, and IL-4 and IL-5 were both undetectable. Compared to nondiseased cats, the diseased population showed a significant increase in the relative mRNA expression of IL-2, IL-4, IL-6, IL-10, IL-12 (p35 and p40), and IFN-gamma. In contrast, IL-5 mRNA expression was unchanged and was only detected in one case. No significant relationship was demonstrable between the change in relative expression of specific cytokine mRNA and the change in clinical severity of the local mucosal lesions over the treatment period. The results demonstrate that the normal feline oral mucosa is biased towards a predominantly (Th) type 1 profile of cytokine expression and that during the development of lesions seen in feline chronic gingivostomatitis there is a shift in the cytokine profile from a type 1 to a mixed type 1 and type 2 response.

Indexed as

AnimalsBiopsyCatsChronic DiseaseCytokinesGene ExpressionGingivitisGlyceraldehyde-3-Phosphate DehydrogenasesInterferon-gammaInterleukin-10Interleukin-12Interleukin-2Interleukin-4Interleukin-5Interleukin-6Mouth MucosaCytokinesGlyceraldehyde-3-Phosphate DehydrogenasesInterferon-gammaInterleukin-10Interleukin-12Interleukin-2Interleukin-4Interleukin-5Interleukin-6RNA, Messenger

Identifiers

PMID10391845
PMCPMC95710
OpenAlexW2115190363

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.