ArticleAntimicrobial agents and chemotherapy1999
Nonactin biosynthesis: the product of nonS catalyzes the formation of the furan ring of nonactic acid.
Article in Antimicrobial agents and chemotherapy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 57 citations in OpenAlex.
- Discovery of a Novel Chromone Enantiomer and the Precursors of Nonactic Acid from the Coral-Reef-DerivedMarine drugs · 2024Article
- Natural products targeting cancer cell dependency.The Journal of antibiotics · 2021Review
- The Structural Diversity of Marine Microbial Secondary Metabolites Based on Co-Culture Strategy: 2009-2019.Marine drugs · 2020Review
- A bacterial biosynthetic pathway for methylated furan fatty acids.The Journal of biological chemistry · 2020Article
- Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster.Microbial cell factories · 2017Article
- Biosynthesis of oxygen and nitrogen-containing heterocycles in polyketides.Beilstein journal of organic chemistry · 2016Review
- Enzymology of Pyran Ring A Formation in Salinomycin Biosynthesis.Angewandte Chemie (International ed. in English) · 2015Article
- Enzymology of Pyran Ring A Formation in Salinomycin Biosynthesis.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2015Article
- Enantiomeric natural products: occurrence and biogenesis.Angewandte Chemie (International ed. in English) · 2012Review
- Alternating pattern of stereochemistry in the nonactin macrocycle is required for antibacterial activity and efficient ion binding.Journal of the American Chemical Society · 2009Article
- Nonactin biosynthesis: setting limits on what can be achieved with precursor-directed biosynthesis.Bioorganic & medicinal chemistry letters · 2009Article
- Genetic modulation of the overexpression of tailoring genes eryK and eryG leading to the improvement of erythromycin A purity and production in Saccharopolyspora erythraea fermentation.Applied and environmental microbiology · 2008Article
- A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genetic localization and molecular characterization of the nonS gene required for macrotetrolide biosynthesis in Streptomyces griseus DSM40695.Antimicrobial agents and chemotherapy · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nonactin is the parent compound of a group of ionophore antibiotics, known as the macrotetrolides, produced by Streptomyces griseus subsp. griseus ETH A7796. Nonactin is a significant compound because of its inhibitory effects on the P170 glycoprotein-mediated efflux of chemotherapeutic agents in multiple-drug-resistant cancer cells. Nonactin is also significant in that it is a highly atypical polyketide. Very little is presently known about the genes of the nonactin biosynthesis cluster. In this paper we describe our efforts to establish a connection between the product of a gene from the nonactin biosynthesis cluster and a known biochemical transformation in nonactin biosynthesis. Nonactate synthase is the enzyme which catalyzes the formation of nonactic acid from an acyclic precursor in nonactin biosynthesis. We have synthesized the substrate for this enzyme and have detected the in vitro cyclization activity of the substrate in cell-free preparations of S. griseus subsp. griseus ETH A7796. Previous studies by R. Plater and J. A. Robinson (Gene 112:117-122, 1992) had suggested, based on sequence homology, that the product of a partial open reading frame found close to the tetranactin resistance gene of S. griseus could be the nonactate synthase. We have therefore cloned, sequenced, and heterologously expressed this full gene (nonS), and we have shown that the gene product, NonS, does indeed catalyze the formation of the furan ring of nonactic acid as hypothesized.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.