ArticleGenetics1999
I-SceI endonuclease, a new tool for studying DNA double-strand break repair mechanisms in Drosophila.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- A CRISPR-Cas9-based system for the dose-dependent study of DNA double-strand break sensing and repair.The FEBS journal · 2025Article
- CellTool: An Open-Source Software Combining Bio-Image Analysis and Mathematical Modeling for the Study of DNA Repair Dynamics.International journal of molecular sciences · 2023Article
- Scarless Modification of the Drosophila Genome Near Any Mapped attP Sites.Current protocols · 2023Article
- pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.FEMS yeast research · 2023Article
- Characterizing the Repair of DNA Double-Strand Breaks: A Review of Surrogate Plasmid-Based Reporter Methods.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide.Journal of microbiology (Seoul, Korea) · 2022Article
- Beta HPV Deregulates Double-Strand Break Repair.Viruses · 2022Review
- The Role ofGenes · 2021Article
- A Targeted and Tuneable DNA Damage Tool Using CRISPR/Cas9.Biomolecules · 2021Article
- Review
- Recombinational Repair of Nuclease-Generated Mitotic Double-Strand Breaks with Different End Structures in Yeast.G3 (Bethesda, Md.) · 2020Article
- Efficient dual-negative selection for bacterial genome editing.BMC microbiology · 2020Article
- Enzyme-Mediated Endogenous and Bioorthogonal Control of a DNAzyme Fluorescent Sensor for Imaging Metal Ions in Living Cells.Angewandte Chemie (International ed. in English) · 2019Article
- Direct observation of end resection by RecBCD during double-stranded DNA break repair in vivo.Nucleic acids research · 2018Article
- Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger.PloS one · 2018Article
- Article
- Quantifying DNA double-strand breaks induced by site-specific endonucleases in living cells by ligation-mediated purification.Nature protocols · 2014Article
- Strategies for gene disruption in Drosophila.Cell & bioscience · 2014Review
- Inducible protein traps with dominant phenotypes for functional analysis of the Drosophila genome.Genetics · 2014Article
- Repair of strand breaks by homologous recombination.Cold Spring Harbor perspectives in biology · 2013Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a step toward the development of a homologous recombination system in Drosophila, we have developed a methodology to target double-strand breaks (DSBs) to a specific position in the Drosophila genome. This method uses the mitochondrial endonuclease I-SceI that recognizes and cuts an 18-bp restriction site. We find that >6% of the progeny derived from males that carry a marker gene bordered by two I-SceI sites and that express I-SceI in their germ line lose the marker gene. Southern blot analysis and sequencing of the regions surrounding the I-SceI sites revealed that in the majority of the cases, the introduction of DSBs at the I-SceI sites resulted in the complete deletion of the marker gene; the other events were associated with partial deletion of the marker gene. We discuss a number of applications for this novel technique, in particular its use to study DSB repair mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.