ArticleGenetics1999
Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 103 citations in OpenAlex.
- Structures and Functions of the Human GATOR1 Complex.Sub-cellular biochemistry · 2024Review
- Hierarchical inhibition of mTORC1 by glucose starvation-triggered AXIN lysosomal translocation and by AMPK.Life metabolism · 2023Article
- Constitutive activation of TORC1 signalling attenuates virulence in the cross-kingdom fungal pathogen Fusarium oxysporum.Molecular plant pathology · 2023Article
- Conserved Pib2 regions have distinct roles in TORC1 regulation at the vacuole.Journal of cell science · 2022Article
- TORC1 and PKA activity towards ribosome biogenesis oscillates in synchrony with the budding yeast cell cycle.Journal of cell science · 2022Article
- Prolonged deprivation of arginine or leucine induces PI3K/Akt-dependent reactivation of mTORC1.The Journal of biological chemistry · 2022Article
- Fine-tuning cell organelle dynamics during mitosis by small GTPases.Frontiers of medicine · 2022Review
- A New Crosslinking Assay to Study Guanine Nucleotide Binding in the Gtr Heterodimer ofSmall GTPases · 2022Article
- The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.iScience · 2021Article
- Review
- Review
- TORC1 signaling regulates cytoplasmic pH through Sir2 in yeast.Aging cell · 2020Article
- Article
- Structural basis for the docking of mTORC1 on the lysosomal surface.Science (New York, N.Y.) · 2019Article
- The NuA4 acetyltransferase and histone H4 acetylation promote replication recovery after topoisomerase I-poisoning.Epigenetics & chromatin · 2019Article
- Signal integration in the (m)TORC1 growth pathway.Frontiers in biology · 2018Article
- Regulation of Sensing, Transportation, and Catabolism of Nitrogen Sources in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2018Review
- Article
- Article
- Pib2 and the EGO complex are both required for activation of TORC1.Journal of cell science · 2017Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prp20p and Rna1p are GDP/GTP exchanging and GTPase-activating factors of Gsp1p, respectively, and their mutations, prp20-1 and rna1-1, can both be suppressed by Saccharomyces cerevisiae gtr1-11. We found that gtr1-11 caused a single amino acid substitution in Gtr1p, forming S20L, which is a putative GDP-bound mutant protein, while Gtr1p has been reported to bind to GTP alone. Consistently, gtr1-S20N, another putative GDP-bound mutant, suppressed both prp20-1 and rna1-1. On the other hand, gtr1-Q65L, a putative GTP-bound mutant, was inhibitory to prp20-1 and rna1-1. Thus, the role that Gtr1p plays in vivo appears to depend upon the nucleotide bound to it. Our data suggested that the GTP-bound Gtr1p, but not the GDP-bound Gtr1p, interacts with itself through its C-terminal tail. S. cerevisiae possesses a novel gene, GTR2, which is homologous to GTR1. Gtr2p interacts with itself in the presence of Gtr1p. The disruption of GTR2 suppressed prp20-1 and abolished the inhibitory effect of gtr1-Q65L on prp20-1. This finding, taken together with the fact that Gtr1p-S20L is a putative, inactive GDP-bound mutant, implies that Gtr1p negatively regulates the Ran/Gsp1p GTPase cycle through Gtr2p.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.