ArticleBiophysical journal1999
Regulation of airway ciliary activity by Ca2+: simultaneous measurement of beat frequency and intracellular Ca2+.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 91 citations in OpenAlex.
- T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection.The Journal of clinical investigation · 2012Trial
- Respiratory Ciliary Beat Frequency in COPD: Balancing Oxidative Stress and Pharmacological Treatment.Antioxidants (Basel, Switzerland) · 2025Review
- Connexin 25 maintains self-renewal and functions of airway basal cells for airway regeneration.Stem cell research & therapy · 2024Article
- Article
- Seeing cilia: imaging modalities for ciliary motion and clinical connections.American journal of physiology. Lung cellular and molecular physiology · 2018Review
- CALHM1-Mediated ATP Release and Ciliary Beat Frequency Modulation in Nasal Epithelial Cells.Scientific reports · 2017Article
- Soluble adenylyl cyclase in health and disease.Biochimica et biophysica acta · 2014Review
- Fluorescence microscopy.Cold Spring Harbor protocols · 2014Review
- An autoregulatory mechanism governing mucociliary transport is sensitive to mucus load.American journal of respiratory cell and molecular biology · 2014Article
- A mechanochemical model for auto-regulation of lung airway surface layer volume.Journal of theoretical biology · 2013Article
- Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography.PloS one · 2013Article
- Neuronal control of pedal sole cilia in the pond snail Lymnaea stagnalis appressa.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2013Article
- Methods to measure and analyze ciliary beat activity: Ca2+ influx-mediated cilia mechanosensitivity.Pflugers Archiv : European journal of physiology · 2012Article
- Intercellular Ca(2+) waves: mechanisms and function.Physiological reviews · 2012Review
- Agonist-evoked Ca(2+) wave progression requires Ca(2+) and IP(3).Journal of cellular physiology · 2010Article
- Role of mechanical stress in regulating airway surface hydration and mucus clearance rates.Respiratory physiology & neurobiology · 2008Review
- In vitro culturing of porcine tracheal mucosa as an ideal model for investigating the influence of drugs on human respiratory mucosa.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2008Article
- TRPV4 channel participates in receptor-operated calcium entry and ciliary beat frequency regulation in mouse airway epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- IP3 sensitizes TRPV4 channel to the mechano- and osmotransducing messenger 5'-6'-epoxyeicosatrienoic acid.The Journal of cell biology · 2008Article
- Differential effects of cyclic and constant stress on ATP release and mucociliary transport by human airway epithelia.The Journal of physiology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Airway ciliary activity is influenced by [Ca2+]i, but this mechanism is not fully understood. To investigate this relationship, ciliary activity and [Ca2+]i were measured simultaneously from airway epithelial ciliated cells. Ciliary beat frequency was determined, for each beat cycle, with phase-contrast optics and high-speed video imaging (at 240 images s-1) and correlated with [Ca2+]i determined, at the ciliary base, by fast imaging (30 images s-1) of fura-2 fluorescence. As a mechanically induced intercellular Ca2+ wave propagated through adjacent cells, [Ca2+]i was elevated from a baseline concentration of 45 to 100 nM, to a peak level of up to 650 nM. When the Ca2+ wave reached the ciliary base, the beat frequency rapidly increased, within a few beat cycles, from a basal rate of 6.4 to 11.6 Hz at 20-23 degrees C, and from 17.2 to 26.7 Hz at 37 degrees C. Changes in [Ca2+]i, above 350 nM, had no effect on the maximum beat frequency. We suggest that airway ciliary beat frequency is 1) controlled by a low range of [Ca2+]i acting directly at an axonemal site at the ciliary base and 2) that a maximum frequency is induced by a change in [Ca2+]i of approximately 250-300 nM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.