ArticleBiophysical journal1999
Caffeine interaction with fluorescent calcium indicator dyes.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 64 citations in OpenAlex.
- Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes.Nature nanotechnology · 2024Article
- Glutathione-Based Photoaffinity Probe Identifies Caffeine as a Positive Allosteric Modulator of the Calcium-Sensing Receptor.ACS chemical biology · 2024Article
- Utilization of the genetically encoded calcium indicator Salsa6F in cardiac applications.Cell calcium · 2024Article
- Sodium-Permeable Ion Channels TRPM4 and TRPM5 are Functional in Human Gastric Parietal Cells in Culture and Modulate the Cellular Response to Bitter-Tasting Food Constituents.Journal of agricultural and food chemistry · 2024Article
- The spatio-temporal properties of calcium transients in hippocampal pyramidal neuronsFrontiers in cellular neuroscience · 2022Article
- Imaging the aged brain: pertinence and methods.Quantitative imaging in medicine and surgery · 2019Review
- A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing.Stem cell reports · 2015Article
- Optical recording of calcium currents during impulse conduction in cardiac tissue.Neurophotonics · 2015Article
- Roles of the NH2-terminal domains of cardiac ryanodine receptor in Ca2+ release activation and termination.The Journal of biological chemistry · 2015Article
- Role of amino-terminal half of the S4-S5 linker in type 1 ryanodine receptor (RyR1) channel gating.The Journal of biological chemistry · 2011Article
- Contributions of SERCA pump and ryanodine-sensitive stores to presynaptic residual Ca2+.Cell calcium · 2010Article
- Axotomy depletes intracellular calcium stores in primary sensory neurons.Anesthesiology · 2009Article
- Tumor necrosis factor-alpha potentiates intraneuronal Ca2+ signaling via regulation of the inositol 1,4,5-trisphosphate receptor.The Journal of biological chemistry · 2008Article
- Store-operated Ca2+ entry during intracellular Ca2+ release in mammalian skeletal muscle.The Journal of physiology · 2007Article
- A mechanical spike accompanies the action potential in Mammalian nerve terminals.Biophysical journal · 2007Article
- Altered energy transfer from mitochondria to sarcoplasmic reticulum after cytoarchitectural perturbations in mice hearts.The Journal of physiology · 2006Article
- Differential sensitivity to perchlorate and caffeine of tetracaine-resistant Ca2+ release in frog skeletal muscle.Journal of muscle research and cell motility · 2006Article
- Caffeine analogs: effects on ryanodine-sensitive calcium-release channels and GABAA receptors.Cellular and molecular neurobiology · 2003Article
- Role of the proposed pore-forming segment of the Ca2+ release channel (ryanodine receptor) in ryanodine interaction.Biophysical journal · 2002Article
- Assessing the role of calcium-induced calcium release in short-term presynaptic plasticity at excitatory central synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We report that caffeine, in millimolar concentrations, interacts strongly with four common calcium indicator dyes: mag-fura-2, magnesium green, fura-2, and fluo-3. Fluorescence intensities are either noticeably enhanced (mag-fura-2, fura-2) or diminished (magnesium green, fluo-3). The caffeine-induced changes in the fluorescence spectra are clearly distinct from those of metal ion binding at the indicator chelation sites. Binding affinities for calcium of either mag-fura-2 or magnesium green increased only slightly in the presence of caffeine. Caffeine also alters the fluorescence intensities of two other fluorescent dyes lacking a chelation site, fluorescein and sulforhodamine 101, implicating the fluorophore itself as the interaction site for caffeine. In the absence of caffeine, variation of solution hydrophobicity by means of water/dioxane mixtures yielded results similar to those for caffeine. These observations suggest that hydrophobic substances, in general, can alter dye fluorescence in a dye-specific manner. For the particular case of caffeine, and perhaps other commonly used pharmacological agents, the dye interactions can seriously distort fluorescence measurements of intracellular ion concentrations with metal indicator dyes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.